Biofuels from algae : methods and protocols

Format:

Book

Contributor:

Spilling, Kristian, editor.

Series:

Methods in molecular biology ; v. 2996.

Methods in molecular biology, 1940-6029 ; 2996

Language:

English

Subjects (All):

Algae--Biotechnology.

Algae.

Algal biofuels.

Physical Description:

1 online resource : illustrations

Edition:

Second edition / edited by Kristian Spilling

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This fully updated edition examines recent advances in algal biotechnology and incorporates both foundational and newly developed protocols for working with algal cultivation systems and biomass. Guiding the reader from initial cultivation to advanced biochemical analysis, the book covers the rationale for using algae in biofuel production and considerations for selecting appropriate strains and cultivation systems, protocols for quantifying key biochemical components of algal biomass, including carbohydrates, lipids, and proteins, as well as what to consider when developing an algal industry such as its environmental impact and what tools and methods can be used in the continued development of this field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls"-- Springer Nature Link

Contents:

Basic methods for isolating and culturing microalgae / Kristian Spilling

Measurement of fluorescence for monitoring algal growth and health / Kristian Spilling, Pasi Ylöstalo and Jukka Seppälä

Large scale cultivation of microalgae in open, closed, and hybrid systems / Jacqueline Nery and Kristian Spilling

Growing algae in biofilms : principles and emerging opportunities / Lara D. Mateos and Kristian Spilling

Combining aquaponics and applied phycology : from bioremediation of wastewater to algal products / Tobias Lipsewers

Monitoring microalgal neutral lipid accumulation with Nile red / Katarina Natunen

Liquid chromatography‑mass spectrometry (LC‑MS)‑based analysis of molecular lipids in algae samples using data dependent acquisition (DDA) / Niels van Boxel, Heli Nygren, Tuulikki Seppänen‑Laakso and Heiko Rischer

UPLC‑ELSD analysis of algal lipid classes and derivatization of bound and free fatty acids and sterols for GC‑MS methods / Tuulikki Seppänen‑Laakso, Heli Nygren and Heiko Rischer

Total fatty acid content determination of whole microalgal biomass using in situ transesterification / Stefanie Van Wychen and Lieve M.L. Laurens

Total carbohydrate content determination of microalgal biomass by acid hydrolysis followed by spectrophotometry or liquid chromatography / Stefanie Van Wychen and Lieve M.L. Laurens

Total protein content determination of microalgal biomass by elemental nitrogen analysis and a dedicated nitrogen‑to‑protein conversion factor / Lieve M.L. Laurens, Jessica L. Olstad and David W. Templeton

Complete acid‑based hydrolysis assay for carbohydrate quantification in seaweed : a species‑specific optimized approach / Emily T. Kostas, Stuart J. Wilkinson, Daniel A. White and David J. Cook

Analysis of brown algal sugars using ion‑exchange high‑performance liquid chromatography / Ingrid Maribu and Jan Arne Arnesen

Chromatographic purification of fucoxanthin from diatom biomass / Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat, Elif Demirkan and Meltem Cokn Dalay

Analytical grade purification of phycocyanin from cyanobacteria / Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat and Meltem Cokn Dalay

Phytoplankton pigment analysis by high performance liquid chromatography / Thomas Rohrlack

Extracellular enzyme activity measurements using fluorescent substrate derivatives / Mari Vahharanta

Determining inorganic and organic carbon / Jaana Koistinen, Mervi Sjöblom and Kristian Spilling

Determining inorganic and organic nitrogen / Jaana Koistinen, Mervi Sjöblom and Kristian Spilling

Total nitrogen determination by a spectrophotometric method / Jaana Koistinen, Mervi Sjöblom and Kristian Spilling

Determining inorganic and organic phosphorus / Jaana Koistinen, Mervi Sjöblom and Kristian Spilling

Determining dissolved and biogenic silica / Jaana Koistinen, Mervi Sjöblom and Kristian Spilling

Microalgae harvesting using fungal mycelia in suspension / Danielle Bangsfield

RNA extraction strategy for algae rich in polysaccharides and polyphenols / Timo Jensen

A simple staining method to study cell structures of microalgae / Tobias Lipsewers

Life cycle assessment of micro and macro algae products / Kitta Silvennoinen, Sara Saukkonen, Jean‑Baptiste Thomas, Léa Braud and Tanja Myllyvirta

Life cycle assessment (LCA) of algal fuels / Homan Hossienadeh‑Bandbafha, Mohammadali Kichhavariandeh, Meisam Tabatabaei, Mortaza Aghbashlo, Alavi Sulaiman and Abbas Ghasemi

Design and analysis of macroalgae‑based biorefineries / Alexander Golberg

Metabolic engineering of microalgae for biofuel production / Mohammad Pooya Naghshbandi, Meisam Tabatabaei, Mortaza Aghbashlo, Muhammad Nauman Aftab and Irfana Iqbal

Notes:

Includes bibliographical references

Online resource; title from PDF title page (Springer Nature Link, viewed May 5, 2026)

Other Format:

Print version: Biofuels from algae

ISBN:

9781071650318

1071650319

OCLC:

1568741188

Publisher Number:

CIPO000325453

Access Restriction:

Restricted for use by site license