Guidance Document on the Diagnosis of Endocrine-related Histopathology in Fish Gonads / OECD.

Format:

Book

Author/Creator:

Organisation for Economic Co-operation and Development, author, issuing body.

Series:

OECD environmental health and safety publications. Series on testing and assessment.

OECD Series on Testing and Assessment

Language:

English

Subjects (All):

Endocrine disrupting chemicals.

Fishes--Diseases.

Fishes.

Physical Description:

1 online resource (114 pages).

Place of Publication:

Paris : OECD Publishing, 2010.

Summary:

Exposure of fathead minnow (Pimephales promelas), Japanese medaka (Oryzias latipes) and zebrafish (Danio rerio) to endocrine disrupting chemicals may result in histopathological alterations of gonadal structure and microarchitecture. The diagnosis of such histopathological changes may, therefore, assist in the detection of endocrine disrupting chemicals. Given the long historical background of piscine histopathology and diverse traditions in the terminology in different fish species and in different regions, there is a need for harmonisation in order to facilitate non-biased comparisons of results from different laboratories and, thus, to optimise reproducibility and reliability of histopathological diagnoses. The purpose of this document is to provide guidance for the preparation and histopathological evaluation of gonads from fathead minnow, Japanese medaka and zebrafish following exposure to chemicals potentially disrupting the endocrine system.

Contents:

ABOUT THE OECD

FOREWORD

Acknowledgements

SUMMARY

1. Purpose of this document

2. Post-mortem and histotechnical procedures

2.1 Fixation of tissue specimens for gonad histopathology

2.2 Euthanasia and necropsy

2.3 Tissue processing

2.4 Embedding

2.5 Microtomy

2.6 Staining, cover-slipping, and slide labeling

3. Gonadal histopathology glossary and diagnostic criteria

3.1 General approach to reading studies

3.2 Diagnostic criteria

3.2.1 Primary criteria in the gonads of male fathead minnow, Japanese medaka and zebrafish

3.2.2 Primary criteria in the gonads of female fathead minnow,

Japanese medaka and zebrafish 3.2.3 Secondary criteria in the gonads of male fathead minnow,

Japanese medaka and zebrafish

3.2.4 Secondary criteria in the gonads of female fathead minnow,

3.3 Severity grading

3.4 Data recording

3.5 Histopathology report format

4. Glossary and diagnostic criteria

4.1 Normal gonadal architecture in fathead minnow, Japanese medaka and zebrafish

4.1.1 Normal testicular architecture in male fathead minnow, Japanese medaka and zebrafish

4.1.2 Normal ovarian architecture in female fathead minnow, Japanese medaka and zebrafish

4.2 Primary diagnoses in fathead minnow, Japanese medaka and zebrafish following exposure to

endocrine disruptors

4.2.1 Primary diagnoses in male fathead minnow, Japanese medaka and zebrafish following

exposure to endocrine disruptors

4.2.2 Primary diagnoses in female fathead minnow, Japanese medaka and zebrafish following

4.3 Secondary diagnoses in fathead minnow, Japanese medaka and zebrafish following exposure

to endocrine disruptors

4.3.1 Secondary diagnoses in male fathead minnow, Japanese medaka and zebrafish following

4.3.2 Secondary in female fathead minnow, Japanese medaka and zebrafish following exposure to

4.4 Additional diagnostic criteria and an illustrated glossary of microanatomical and diagnostic

terms

5. Gonadal staging criteria

5.1 Criteria for staging testes in fathead minnow and zebrafish

5.2 Criteria for staging testes in Japanese medaka

5.3 Criteria for staging ovaries in fathead minnow, Japanese medaka and zebrafish

6. References.

Notes:

Description based on publisher supplied metadata and other sources.

ISBN:

92-64-82565-7