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Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test [electronic resource] / Organisation for Economic Co-operation and Development
- Format:
- Book
- Government document
- Author/Creator:
- Organisation for Economic Co-operation and Development.
- Series:
- OECD Guidelines for the Testing of Chemicals, Section 2, 20745761.
- Language:
- English
- Subjects (All):
- Chemical tests and reagents--Handbooks, manuals, etc.
- Chemical tests and reagents.
- Freshwater algae.
- Cyanobacteria.
- Physical Description:
- 1 online resource (25 pages)
- Place of Publication:
- Paris : OECD Publishing, 2011.
- Language Note:
- English
- Summary:
- The purpose of this test is to determine the effects of a substance on the growth of freshwater microalgae and/or cyanobacteria. Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 hours. The system response is the reduction of growth in a series of algal cultures exposed to, at least, five concentrations of a test substance. Three replicates at each test concentration should be used. The response is evaluated as a function of the exposure concentration in comparison with the average growth of control cultures. The cultures are allowed unrestricted exponential growth under nutrient sufficient conditions (two alternative growth media: the OECD and the AAP) and continuous fluorescent illumination. Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. The limit test corresponds to one dose level of 100 mg/L. This study includes: the determination, at least daily, of the algal biomass; the measure of the pH (at the beginning and at the end); microscopic observation. This Test Guideline describes two response variables: average specific growth rate, and yield.
- Notes:
- Bibliographic Level Mode of Issuance: Monograph
- ISBN:
- 1-280-60753-X
- 9786610607532
- 92-64-06992-5
- OCLC:
- 1024285398
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