Aspergillus fumigatus : methods and protocols / edited by Natalia Mach

Format:

Book

Contributor:

Mach, Natalia, editor.

Series:

Methods in molecular biology, 1940-6029 ; 3028

Language:

English

Subjects (All):

Aspergillus fumigatus.

CRISPR (Genetics).

Medical mycology.

Clustered Regularly Interspaced Short Palindromic Repeats.

Medical Subjects:

Aspergillus fumigatus.

Clustered Regularly Interspaced Short Palindromic Repeats.

Physical Description:

1 online resource : illustrations (some color)

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This book presents current molecular biology methods involving Aspergillus fumigatus for researchers in mycology, fungal genetics, and medical microbiology looking to deepen their understanding of this species of fungus at the molecular level. Sections of the book explore approaches to CRISPR-based genome editing, methods for identification, genotyping, and detection, assays for gene regulation, protocols for electrophoretic mobility shift assays and chromatin immunoprecipitation, as well as models to study host-pathogen interactions and methods to investigate the fungal cell wall and secreted components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Aspergillus fumigatus: Methods and Protocols serves as an ideal guide for scientists attempting to shorten the time spent on routine problems at the bench and move more quickly from idea to results"-- Springer Nature Link

Contents:

Cas9 ribonucleoproteins (RNPs) for gene deletion / Adela Martin-Vicente and Jarrod R. Fortwendel

Generation of seamless point mutations with Cas9 RNP and pTel-hygR plasmid in Aspergillus fumigatus / Mariana Handelman, Hila Werner, and Nir Osherov

Marker-free CRISPR/Cas9 RNP transformation for gene disruption in Aspergillus fumigatus / Clara Valero, Jamie Tindale, Michael J. Bromley, and Norman van Rhijn

CRISPR-Cas9 and microhomology-mediated end-joining for biosynthetic pathway reconstruction in Aspergillus fumigatus / Yaojie Guo and Daniel H. Scharf

PCR typing and genome sequencing of Aspergillus fumigatus / Hiroki Takahashi, Saho Shibata, and Momotaka Uchida

Isolation of nucleic acids from Aspergillus fumigatus for sequencing / Sam El-Kamand and Charles Oliver Morton

Methods for large-scale intraspecific analyses of single nucleotide polymorphisms in the mitochondrial genomes of Aspergillus fumigatus / Veronica Thorn and Jianping Xu

Development and validation of a real-time PCR assay for the detection of Aspergillus fumigatus on Panther Fusion system / Margherita Cacaci, Damiano Squitieri, Maurizio Sanguinetti, and Brunella Posteraro

RAPD-PCR for typing and identification of Aspergillus fumigatus / María del Rocío Reyes-Montes, Esperanza Duarte-Escalante, and María Guadalupe Frías-De-León

Electrophoretic mobility shift assay in Aspergillus fumigatus / Vijendra Arya, Sanjoy Paul, and W. Scott Moye-Rowley

Chromatin immunoprecipitation in Aspergillus fumigatus / Sanjoy Paul, Mark A. Stamnes, and W. Scott Moye-Rowley

Micropatterning substrates using deep UV lithography : a model to study host–pathogen interactions of A. fumigatus with adherent cells / Julien Polleux, Lea Heinrich, Susanne Perkhofer, and Natalia Mach

Bronchoalveolar lavage fluid collection in invasive pulmonary aspergillosis murine model for downstream proteomic analysis / Sarah Sze Wah Wong

Galleria mellonella to study Aspergillus infections and antifungal efficacy by traditional readouts and novel BL-imaging techniques / Jakob Scheler and Ulrike Binder

Molecular biosensing of the Aspergillus fumigatus cell wall integrity pathway using the (p)agsA::luc (luciferase) reporter system / Jonatas Erick Maimoni Campanella, Mark Arentshorst, Arthur F. J. Ram, Cees A. M. J. J. van den Hondel, and Iran Malavazi

Evaluation of Aspergillus fumigatus metabolic activity in vitro : a comparison between resazurin and XTT methods / Thaila Fernanda dos Reis, Iran Malavazi, and Gustavo Henrique Goldman

Aspergillus fumigatus cell wall : current advances in structural elucidation methods / Chaithanya Shetty, Melba Elizabeth-Biju, and Vishukumar Aimanianda

Isolation and purification of extracellular vesicles from Aspergillus fumigatus / Lucas F. B. Nogueira and Fausto Almeida

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed April 16, 2026)

Other Format:

Print version: Aspergillus fumigatus

ISBN:

9781071652220

1071652222

OCLC:

1585500713

Publisher Number:

CIPO000366816

Access Restriction:

Restricted for use by site license