Development and (Evidence for) Destruction of Biofilm with Pseudomonas aeruginosa as Architect Department of Chemistry State University of New York Binghamton, NY

Format:

Conference/Event

Author/Creator:

Uzcategui, Valerie N., author.

Conference Name:

International Conference On Environmental Systems (1991-07-08 : San Francisco, California, United States)

Language:

English

Physical Description:

1 online resource

Place of Publication:

Warrendale, PA SAE International 1991

Summary:

AbstractDisinfection and maintenance of an acceptable level of asepsis in spacecraft potable water delivery systems is a formidable task. The major area of research for this project has been to monitor the formation and growth of biofilm, and biofilm attached microorganisms, on stainless steel surfaces (specifically coupons), and the use of ozone for the elimination of these species in a closed loop system.A number of different techniques have been utilized during the course of a typical run. Scraping and sonication of coupon surfaces with subsequent plating as well as epifluorescence microscopy have been utilized to enumerate biofilm protected Pseudomonas aeruginosa. In addition, scanning electron microscopy is the method of choice to examine the integrity of the biofilm. For ozone determinations, the indigo decolorization spectrophotometric method seems most reliable. Both high- and low-nutrient cultured P. aeruginosa organisms were the target species for the ozone disinfection experiments

Notes:

Vendor supplied data

Publisher Number:

911404

Access Restriction:

Restricted for use by site license