Toxicological Impact of Diesel Fuel Supplementation with Rapeseed Methylester (RME) on the Lung Toxic Potential of Diesel Engine Exhausts VACOMED-INSERM E9920

Format:

Conference/Event

Author/Creator:

Le Prieur, Elsa, author.

Conference Name:

CEC/SAE Spring Fuels & Lubricants Meeting & Exposition (2000-06-19 : Paris, France)

Language:

English

Physical Description:

1 online resource

Place of Publication:

Warrendale, PA SAE International 2000

Summary:

Organotypic cultures of precision-cut rat lung slices are exposed three hours to 5 dilutions of a continuous exhaust flow. The exhausts from three fuelings are tested (filtered and not): gazole, gazole/RME mixture (70 % / 30%) and RME.Different parameters are assessed: ATP and GSH (cell viability), TNFα production (proinflammatory response) and nucleosomes (apoptosis). After exposure, the ATP level is only modified by the highest concentrations of gazole/RME or RME exhausts (filtered and not); the GSH level is decreased for each of the concentrated filtered exhausts. Moreover, only the whole gazole exhaust leads to a modification of TNFα production. At last, the whole gazole exhaust leads to an increase of slice nucleosome level in a dose-dependent way; RME supplementation or filtration significantly attenuates this effect.In conclusion, whole diesel exhaust induces an inflammatory response and DNA alterations, which are partially or totally reversed by filtration and/or by RME supplementation

Notes:

Vendor supplied data

Publisher Number:

2000-01-2060

Access Restriction:

Restricted for use by site license