Microfabricated Devices for <em>In-Vivo</em> Sensing for Mitochondrial Assessment Vishal Venkatesh

Format:

Book

Thesis/Dissertation

Author/Creator:

Venkatesh, Vishal, author.

Contributor:

University of Pennsylvania. Electrical and Systems Engineering., degree granting institution.

Language:

English

Subjects (All):

0202.

0537.

0719.

Local Subjects:

0202.

0537.

0719.

Physical Description:

1 electronic resource (165 pages)

Contained In:

Dissertations Abstracts International 87-07B

Place of Publication:

Ann Arbor : ProQuest Dissertations and Theses, 2025

Language Note:

English

Summary:

Monitoring of oxygen concentration in biological tissues is essential for understanding cellular metabolism, mitochondrial function, and tissue regeneration. Mitochondrial dysfunction is implicated in a wide range of metabolic and degenerative diseases, yet current diagnostic approaches lack the capability to continuously measure oxygen and metabolite dynamics in vivo. This work introduces an implantable electrochemical sensor platform developed for real-time monitoring of tissue oxygen tension to support diagnosis and study of mitochondria-related disorders. The research encompasses device design, fabrication, preclinical validation, and clinical testing within a translational framework. The electrochemical oxygen sensor employs a hydrogel-based electrolyte that enhances mechanical stability, prevents leakage, and supports long-term function under physiological conditions. Devices are fabricated through a scalable and simplified process on flexible substrates and integrated with an oxygen-permeable polydimethylsiloxane membrane for selective gas diffusion. Electrochemical characterization using linear sweep voltammetry confirmed stable and reproducible sensor response across physiological oxygen concentrations. In vivo validation using zebrafish muscle models demonstrated reliable detection of intramuscular oxygen tension during controlled activity tests, while clinical evaluation in human subjects established feasibility for continuous monitoring during rest and exercise. Comparative sterilization studies identified Sterrad sterilization as the optimal method for preserving sensor functionality, showing minimal post-process degradation and stable performance following rehydration. To address mechanical and deployment challenges, sensor miniaturization was implemented using a dip-coating process for uniform membrane formation and optimized hydrogel electrolyte formulation for consistent sensitivity. To extend diagnostic capability, a complementary lactate sensor was also developed using lactate oxidase-based enzymatic detection. Together, these oxygen and lactate sensors establish a foundation for multiplexed, implantable metabolic monitoring platforms suited for continuous in vivo assessment of mitochondrial function

Notes:

Advisors: Allen, Mark G. Committee members: Olsson, Troy; Issadore, David; Zolkipli-Cunningham, Zarazuela

Source: Dissertations Abstracts International, Volume: 87-07, Section: B.

Ph.D. University of Pennsylvania 2025

Vendor supplied data

Local Notes:

School code: 0175

ISBN:

9798276006451

Access Restriction:

Restricted for use by site license