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Design of Photoactivatable Chemically Induced Proximity Probes: Extending Beyond Two-Protein Interactions Changfeng Deng
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Deng, Changfeng, author.
- Language:
- English
- Subjects (All):
- 0307.
- 0485.
- 0487.
- Local Subjects:
- 0307.
- 0485.
- 0487.
- Physical Description:
- 1 electronic resource (227 pages)
- Contained In:
- Dissertations Abstracts International 87-07B
- Place of Publication:
- Ann Arbor : ProQuest Dissertations and Theses, 2025
- Language Note:
- English
- Summary:
- Chemically induced dimerization (CID) is a versatile molecular strategy that enables the controlled assembly of two proteins in response to a small-molecule dimerizer. When integrated with photoresponsive functional groups, photoactivatable chemically induced dimerization (photo-CID) emerges as a powerful technique for achieving spatiotemporal control over protein-protein interactions using light as an external trigger. This added layer of optical control allows researchers to precisely modulate molecular events in living systems with high temporal resolution and subcellular specificity. To further expand the molecular toolset of these systems, we developed strategies to incorporate a third protein into the interaction network. These include dual-target dimerizers, which promote the formation of both binary and ternary protein complexes, and swapper molecules, which enable dynamic switching between binary protein interactions among three proteins. Additionally, we introduced the concept of conditional localization pharmacology, a strategy designed to harness drug activity in a more spatially and functionally specific manner. Together, these innovations provide a modular platform with expanded capabilities, enabling programmable and light-controllable manipulation of more complex and dynamic protein interaction networks in living cells
- Notes:
- Advisors: Chenoweth, David M. Committee members: Petersson, M. James; Huryn, Donna; Vinogradov, Sergei
- Source: Dissertations Abstracts International, Volume: 87-07, Section: B.
- Ph.D. University of Pennsylvania 2025
- Vendor supplied data
- Local Notes:
- School code: 0175
- ISBN:
- 9798276001579
- Access Restriction:
- Restricted for use by site license
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