Stem cell therapy : practical considerations / Hala M. Gabr and Wael Abo El-Kheir ; scientific illustrator, Abdullah Mahmoud.

Format:

Book

Author/Creator:

Gabr, Hala M., author.

El-Kheir, Wael Abo, author.

Language:

English

Subjects (All):

Stem cells--Therapeutic use.

Stem cells.

Stem cells--Transplantation.

Stem Cell Transplantation.

Stem Cells.

Medical Subjects:

Stem Cell Transplantation.

Stem Cells.

Physical Description:

1 online resource (166 pages)

Place of Publication:

London, England ; San Diego, California : Elsevier, [2023]

Summary:

Stem cell therapy is a fast-growing field of medicine with remarkable prospects in a broad spectrum of diseases. Stem Cell Therapy: Practical Considerations addresses the biological properties of stem cells, mechanisms of action; as well as actual therapeutic decisions such as cell type, source, dose, manipulation, and route of injection.

Contents:

Front Cover

Stem Cell Therapy

Copyright Page

Dedication

Contents

I. Stem cells: basic biology

1 Introduction

1.1 Establishing the concept of stem cells

Further reading

2 Anatomy and Histology

2.1 Blood supply

2.2 Niche

2.3 Hemopoietic stem cell niches

2.4 Differences between osteoblastic and vascular niches

2.5 Components of the hemopoietic stem cell niche

2.6 Mesenchymal stem cell niche

2.6.1 Bone marrow mesenchymal stem cell niche

2.7 Mesenchymal stem niche in adipose tissue

2.7.1 Epidermal stem cell niche

2.7.2 Intestinal epithelial niche

2.8 Niche functions

2.9 Regulation of niche function

3 Stem cells: definition, biological types, classifications, and properties

3.1 Definition

3.2 Classification

3.3 Properties

3.3.1 Potency

3.3.1.1 Totipotent stem cells

3.3.1.2 Pluripotent stem cells

3.3.1.3 Molecular determinants of pluripotency

3.3.1.4 Cell cycle profile of pluripotency

3.3.1.5 Multipotent stem cells

3.3.1.6 Oligopotent stem cells

3.3.1.7 Bipotent stem cells

3.3.1.8 Unipotent stem cells

3.3.2 Self-renewal

3.3.2.1 Regulation of self-renewal

3.3.3 Stem cell plasticity

3.3.4 Evidence of stem cell transdifferentiation

3.3.5 Theories of transdifferentiation

3.3.5.1 Requirements for proof of transdifferentiation

3.3.5.2 Factors affecting frequency of transdifferentiation

3.3.5.3 Induction of transdifferentiation

3.3.5.4 Evaluation of transdifferentiation

3.4 Differentiation

3.5 Homing and engraftment

3.5.1 Definition

3.5.2 Mechanism of homing

3.5.3 Enhanced and directed homing

3.5.4 Stem cell trafficking

3.5.5 Mechanism of stem cell trafficking

3.5.6 Role of stem cell trafficking in regeneration

Further reading.

II. Stem cell induced regeneration: principles and applications

4 Mechanisms of stem cell-related regeneration

4.1 Regulation of tissue regeneration

4.2 Overview of the mechanism of regeneration

4.2.1 Transdifferentiation

4.2.2 Contribution to tissue regeneration

4.2.3 Variation in transdifferentiation potential

4.2.4 Molecular mechanisms of transdifferentiation

4.3 Signaling pathways involved in stem cell transdifferentiation

Mogrify algorithm

4.4 Activation of endogenous repair system

4.5 Induction of angiogenesis

4.5.1 Variability in angiogenic potential of mesenchymal stem cells

4.6 Secretory function

4.6.1 Microvesicles and exosomes

4.6.2 Paracrine action

4.7 Mitochondrial transfer

4.7.1 Inducers of transfer

4.7.2 Mechanisms

4.7.3 Effects

4.7.4 Variability in mitochondrial transfer according to mesenchymal stem cell source

4.8 Microenvironment modulation

4.9 Conclusion

5 Sources of stem cells for regenerative purposes

5.1 Characteristics of the ideal source of stem cells

5.2 Sources of mesenchymal stem cells

5.3 Skin mesenchymal stem cells

5.4 Periodontal ligament stem cells

5.4.1 Sources of hemopoietic stem cells

5.5 Cord blood

5.5.1 Sources of endothelial cells

5.6 Tissue-specific stem cells

6 Basic concepts in stem cell therapy: stem cell therapy strategy development

6.1 Introduction

6.2 What is the basic defect?

6.2.1 Cellular defects

6.2.2 Structural defects

6.3 Which cell lineage is the best for repair? Choosing optimum cell lineage

6.4 What is the best source for a cell type? Choosing the optimum cell source

6.4.1 Hemopoietic stem cells

6.4.2 Mesenchymal stem cells

6.5 What is the optimum laboratory protocol?

6.6 Choosing the optimum route of injection.

6.7 Factors affecting homing of stem cells

6.7.1 Methods to improve stem cell homing

6.7.2 Mesenchymal stem cell homing

6.8 Optimal timing for cell therapy

6.9 Optimum number of cells for injection

6.10 Choosing the method of evaluation

III. Guidelines for stem cell therapy

7 Stem Cell Therapy: Laboratory guidelines

7.1 Introduction

7.1.1 Regulatory bodies

7.1.1.1 Stem cell therapy types in regard to manipulation

7.2 Laboratory guidelines

7.2.1 Structural facilities

7.2.1.1 Good tissue practice

7.2.1.1.1 Good tissue practice environmental control

7.2.1.2 Good manufacturing practice

7.2.1.2.1 Facilities

7.2.1.2.2 Equipment

7.2.1.2.3 Basic equipment for stem cell laboratory are

7.2.1.2.4 Supplies and reagents

7.2.1.2.5 Processing and process control

7.2.2 Personnel qualifications and job description

7.2.2.1 Administrative team

7.2.2.2 Ethics team

7.2.2.3 Clinical evaluation team

7.2.2.4 Stem cell collection team

7.2.2.5 Stem cell handling team (laboratory)

7.2.2.6 Clinical followup team

7.3 Quality control team

8 Protocol guidelines for clinical decisions in stem cell therapy

8.1 Introduction

8.1.1 Timing of intervention

8.1.2 Stem cell injection

8.1.2.1 Route of injection

8.1.3 Frequency of injection

8.1.3.1 Methods of evaluation

8.1.3.1.1 Patient

8.1.3.1.2 Cells

Reference

9 Protocol guidelines: laboratory decisions in stem cell therapy

9.1 Introduction

9.2 Choosing the cell purification level

9.3 Choice of purification strategy

9.4 Choice of stem cell differentiation status

9.5 Choice of cell dose

9.6 Choice of tracking method

9.6.1 Methods of in vivo cell tracking

9.6.1.1 Experimental

9.6.1.2 Methods of in vivo tracking in humans.

10 Phases of translational stem cell research

10.1 Introduction

10.2 Laboratory experiments

10.3 Preclinical, experimental

10.3.1 Value of animal experiments

10.3.1.1 Animal models

10.3.1.2 Species selection

10.3.1.3 Strain selection

10.3.1.4 Methods of inducing animal models

10.3.2 Small versus large animals

10.3.3 Challenges in animal experiments

10.3.4 Ethical considerations

10.4 Clinical trials

11 Ethical guidelines of stem cell therapy

11.1 Introduction

11.1.1 Ethical guidelines for the use of embryonic cells

11.1.2 Ethical concerns

11.1.3 Ethical guidelines for preclinical studies

11.1.4 Principles of ethical guidelines of clinical translation

12 Stem cell therapy: practical examples

12.1 Neuroregeneration

12.1.1 In vitro experiments

12.2 Experimental animal study

12.2.1 Induction of animal model

12.2.2 Stem cell therapy

12.2.3 Followup

12.3 Results

12.3.1 Clinical translational study

12.4 Results of clinical translational study

12.5 Replacement of defective protein

12.5.1 Epidermolysis bullosa

12.6 Results

12.7 Multifactorial defects

12.7.1 Results

12.7.1.1 In vitro experiments

12.7.1.2 Clinical results

12.8 Conclusion

Appendix A

A.1 Standard operating procedures for stem cell therapy procedures

A.1.1 Collection

A.1.1.1 Collection of peripheral blood hemopoietic stem cells

A.1.1.2 Collection of bone marrow hemopoietic stem cells

A.1.1.3 Cord blood stem cell collection

A.1.1.4 Collection of stem cells from adipose tissue

A.1.2 Isolation

A.1.2.1 Mononuclear cell separation by density gradient centrifugation

A.1.2.2 Mesenchymal Stem Cell Isolation from Wharton Jelly

A.1.3 Mesenchymal stem cell culture

A.1.3.1 Identification of mesenchymal stem cells.

A.1.4 Differentiation

A.1.4.1 Plating cells for differentiation

A.1.4.2 Evaluation of differentiation

A.1.5 Coating of flasks

A.1.6 Labeling

Index

Back Cover.

Notes:

Includes bibliographical references and index.

Description based on print version record.

Other Format:

Print version: Gabr, Hala M. Stem Cell Therapy

ISBN:

9780128215708

0128215704