Euglenozoa methods and protocols Volume 2 edited by Paul A.M. Michels, Michael L. Ginger, Anna Karnkowska, Laura-Isobel McCall, Ariel M. Silber

Format:

Book

Contributor:

Michels, Paul A. M., editor.

Ginger, Michael L., editor.

Karnkowska, Anna, editor.

McCall, Laura-Isobel, editor.

Silber, Ariel M., editor.

Series:

Methods in molecular biology ; v. 3014.

Methods in molecular biology 1940-6029 3014

Language:

English

Subjects (All):

Flagella (Microbiology).

Flagella.

Medical Subjects:

Flagella.

Physical Description:

1 online resource

Place of Publication:

New York, NY Humana Press [2026]

Summary:

"This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 2 presents three sections exploring techniques in molecular biology, cell biology, as well as biochemistry and physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 2 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology"-- Springer Nature Link

Contents:

Genetic manipulation of Paradiplonema papillatum / Drahomíra Faktorová, Matus Valach, Orsola Iorillo, Bungo Akiyoshi, Gertraud Burger, Julius Lukes

Protocol for efficient ribodepletion of Euglena gracilis RNA / Natalia Guminska, Pawel Halakuc, Bozena Zakrys, Rafal Milanowski

Engineering wax ester composition in Euglena gracilis using genome editing / Sakura Nagamine, Rikuto Oishi, Masami Nakazawa

Multi-approach workflows for chromatin and histone isolation from Trypanosoma cruzi : From basic protein extraction to mass spectrometry-compatible protocols / Ana Paula Menezes, Julia Pinheiro Chagas da Cunha

Precision without selection : A marker-free CRISPR/Cas9-based protocol for multiplexed genome editing in trypanosomatids / Kathyanna Arnould, Mohammad El Kadri, Perrine Hervé, Corinne Asencio, Nicolas Plazolles, Sarah Monic, Chloé Alexandra Morel, Loïc Rivière, Frédéric Bringaud, Emmanuel Tetaud

Application of double-inducible expression system in bloodstream-form Trypanosoma brucei / Lisa Hohnen, Ralf Erdmann, Vishal C. Kalel

Tetracycline-inducible functional complementation analysis in Trypanosoma parasites / Chethan K. Krishna, Ralf Erdmann, Vishal C. Kalel

Immunoprecipitation of protein complexes from Trypanosoma brucei / Aleksandra Ciszek, Midori Ishii, Bungo Akiyoshi

Standardized recombinase polymerase amplification (RPA) assay for detecting active Trypanosoma brucei evansi infections / Zeng Li, Andrés Álvarez-Rodríguez, Yann G.-J. Sterckx, Stefan Magez

High-throughput cell cycle and morphological analysis of Leishmania mexicana and other kinetoplastids / Jessie Howell, Melanie Jimenez, Tansy C. Hammarton

Protein localization with streptavidin-imaging : Boost your signal, reduce noise, overcome antibody accessibility problems, and time-travel to the past / Johanna Krenzer, Bernardo Papini Gabiatti, Silke Braune, Siqi Shen, Martin Zoltner, Susanne Kramer

A practical guide to ultrastructural expansion microscopy (U-ExM) in Trypanosoma brucei / Bernardo Papini Gabiatti, Athina Paterou, Silke Braune, Johanna Krenzer, Luka-Michael Jekic, Benjamin Dietz, Samuel Dean, Susanne Kramer

Expansion microscopy in Euglenozoa / Marie Zelená, Anežka Konupková, Vladimír Hampl, Vladimír Varga

Colab-PEX19BS : An automated Google Colab notebook-based tool for detecting PEX19-binding sites / Samantha Jeng, Chethan K. Krishna, Ralf Erdmann, Vishal C. Kalel

Biochemistry of diplonemids / Matus Valach, Gertraud Burger, Drahomíra Faktorová, Julius Lukes, Ingrid Škodová-Šveráková

Euglena gracilis subcellular fractionation / Lucia Tomecková, Vladimír Hampl, Priscila Peña-Diaz

Isolation of mitochondria and chloroplasts from Euglena gracilis Z / Shun Tamaki, Takahiro Ishikawa

Imaging Ca2+ signaling in Trypanosoma cruzi with genetically encoded Ca2+ indicators / Guozhong Huang, Mayara Bertolini, Roberto Docampo

Enzyme assays for phosphoinositide kinases and phosphatases and inhibitor screens / Mengjin Gao, Manoj Koirala, Natalie B. Warren, Jacqueline C. Bede, Igor Cestari

Noninvasive, fluorescence-based detection of perturbations in the thiol-redox homeostasis of bloodstream Trypanosoma brucei brucei / Florencia Sardi, Cristina Quiroga, Natalia Oddone, Marcelo A. Comini

Assessment of mitochondrial membrane potential in intact and detergent-permeabilized Trypanosoma brucei insect and bloodstream forms / Alena Zíková, Michaela Husová, Ayyüce Sever, Michaela Kunzová, Eva Doleželová

Camelid single-domain antibodies as tools for novel target identification in kinetoplastid research / Steven Odongo, Line De Vocht, Bo-kyung Jin, Zeng Li, Stefan Magez, Yann G.-J. Sterckx

Method for purification and electrophysiological recordings of ion channels from trypanosomatids / Veronica Jimenez

Functional studies of kinetoplastid transporters / Harry P. de Koning

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed February 9, 2026)

Other Format:

Print version Euglenozoa

ISBN:

9781071651469

1071651463

OCLC:

1572073932

Access Restriction:

Restricted for use by site license