Extracellular vesicles / edited by Amit K. Srivastava.

Format:

Book

Contributor:

Srivastava, Amit K., editor.

Series:

Neuromethods ; 228.

Neuromethods, 1940-6045 ; 228

Language:

English

Subjects (All):

Extracellular space.

Physical Description:

1 online resource (xiv, 380 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume presents the latest advancements in extracellular vesicles (EV) research, drawing on the expertise of investigators across disciplines and institutions. The chapter in this book cover a wide array of experimental approaches, from foundational isolation and enrichment techniques to cutting-edge analytical tools and therapeutic applications. Chapter topics include tangential flow filtration for large-scale isolation, magnetic bead-based separation, and optimized methods for recovering EVs from diverse biological sources. Some chapter also cover specialized procedures that detail the capture of EV surface proteins, electron tomography-based structural analysis, and innovative strategies to load small RNAs into EVs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Extracellular Vesicles serves as a practical and accessible reference for both new and experienced researchers. Whether working with clinical samples, developing therapeutic EVs, or studying their basic biology, investigators will find detailed protocols accompanied by critical commentary on experimental design, controls, and interpretation"-- Springer Nature Link.

Contents:

Extracellular vesicles: Biology, functions, and applications / Sowmya Shree Gopal, Mandeep Kaur, Sophie Lanzkron, and Amit K. Srivastava

A protocol to capture the extracellular vesicle surface (surfaceome) combining biotinylation and mass spectrometry / Iasmin Inocencio, Alin Rai, and David W. Greening

Standardized protocol for magnetic bead-based enrichment and separation of extracellular vesicles / Ketil Winther Pedersen and Axl A. Neurauter

Isolation of CNS-originating extracellular vesicles from blood and cell culture media / Ibrar Siddique, Megan Hong, Kimia Mohseni, Seyedshahabadin Bidoki, and Gal Bitan

Isolation of spontaneously released extracellular vesicles from human and mouse brain / Patrícia A. Gomes, Kalliopi Skourti, Filippos Katsaitis, Cristian Bodo, Minghao Chen, Clarissa L. Waites, Christos Gatsogiannis, Nuno Sousa, Bruno Costa-Silva, and Ioannis Sotiropoulos

Methods for isolating and characterizing red blood cell extracellular vesicles / Gurneet S. Sangha, Marzyeh Kheradmand, Lauren V. Smith, and Alisa Morss Clyne

Analysis of urinary extracellular vesicles / Uta Erdbrügger, Martin E. van Royen, Gerald W. Verhaegh, Cristina Grange, Dylan Burger, Morgan Greig, Joshua A. Welsh, Tuula Anneli Nyman, Alicia Llorente, Elena Martens-Uzunova, Maija Puhka, Karina A. Barreiro, and Monica Suet Ying Ng

From conditioned media to therapeutics: Isolation and characterization of mesenchymal stem cell–derived extracellular vesicles / Chippy K. Mathew, Yashvi Sharma, and Sujata Mohanty

Large-scale isolation of extracellular vesicles using tangential flow filtration method / Mandeep Kaur, Sowmya Shree Gopal, Zenia Barretto, and Amit K. Srivastava

Optimized buffer conditions for storage and recovery of extracellular vesicles / André Görgens and Samir El Andaloussi

Nanoparticle tracking analysis and its application to study the role of small extracellular vesicles in health and disease / Ella Fouhy, Daniel O’Reilly, and Patricia B. Maguire

Innovative methods to label, track, and quantitate extracellular vesicle uptake / Amritha Achuthkumar, Peter J. Wermuth, and M. G. Mahoney

Protocol for assessing the procoagulant and prothrombotic potential of extracellular vesicles / Kimberly A. Thomas, Baron K. Osborn, and Jessica C. Cardenas

Electron tomography-based reconstruction of extracellular vesicles in tissue / Massimo Micaroni, Jens Berndtsson, Ornella Urzì, and Rossella Crescitelli

Strategies for loading miRNA cargo into extracellular vesicles / Nico G. Menjivar, Camila Azzolin de Souza, Ahmed Gad, Luca A. Souza, Samuel Gebremedhn, Juliano C. da Silveira, and Dawit Tesfaye

Bioinformatics tools and databases for extracellular vesicles and particles research / Sriram Gummadi, Sai V. Chitti, Suresh Mathivanan, Sanjay Shahi, and Pamali Fonseka.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed January 29, 2026).

Other Format:

Print version: Extracellular vesicles

ISBN:

9781071649053

1071649051

OCLC:

1570402509

Access Restriction:

Restricted for use by site license