Influence of Physical Cues from the Degrading Magnesium Implants on Human Cells.

Format:

Book

Author/Creator:

Amberg, Romina.

Language:

English

Subjects (All):

Guided bone regeneration.

Biomedical materials.

Physical Description:

1 online resource (48 pages)

Edition:

1st ed.

Place of Publication:

Hamburg : Diplomica Verlag, 2025.

Summary:

This book explores the impact of degrading magnesium implants on human cells, focusing on their application in guided bone regeneration (GBR) to treat periodontal defects in dentistry. It examines the migration, viability, and proliferation of human gingival fibroblasts (HGF) on magnesium surfaces compared to other materials like titanium and plastic. The study investigates physical cues such as altered magnesium and calcium ion concentrations, hydrogen release, and osmolality changes resulting from magnesium corrosion. It provides insights into optimizing magnesium membranes for clinical applications, aiming to improve healing success in dental and orthopedic procedures. Intended for researchers and professionals in biomaterials, bioengineering, and medical sciences. Generated by AI.

Contents:

Cover

Copyright

Table of Contents

Abstract

Zusammenfassung

1 Introduction

2 Methods

2.1 Cell culture

2.1 Migration assay

2.2 Surface characterization

2.2.1 Topography - Scanning electron microscopy (SEM)

2.2.2 Roughness - Atomic force microscopy (AFM)

2.2.3 Wettability measurements

2.3 Modified migration assay

2.4 Characterization of the supernatants during cell migration

2.5 Ca2+ determination in the corrosion layer of Mg membranes

2.6 Immunohistological analysis

2.7 Preparing test solutions

2.8 Toxicological assays (MTT, BrdU) with test solutions

2.9 Migration assay with test solutions

2.10 Data and statistical analysis

3 Results

3.1 Migration behaviour on Mg, Ti and TCP

3.2 Characterized Mg, Ti and TCP surfaces

3.3 Mg2+ affects cell adhesion

3.4 Unmodified vs. modified migration assay (Mg-direct/ Mgindirect)

3.5 Characterized supernatants during cell migration

3.6 Ca2+ storage in the corrosion layer of Mg

3.7 Effect of Mg2+ on cells

3.8 Effect of Ca2+ on cells

3.9 Effect of H2 on cells

3.10 Effect of Mg extracts on cells

4 Discussion

5 Literature.

Notes:

Description based on publisher supplied metadata and other sources.

Part of the metadata in this record was created by AI, based on the text of the resource.

ISBN:

9783959356152

OCLC:

1526859854