Light sheet microscopy / edited by Ludovico Silvestri.

Format:

Book

Contributor:

Silvestri, Ludovico, editor.

Series:

Neuromethods ; 229.

Neuromethods, 1940-6045 ; 229

Language:

English

Subjects (All):

Fluorescence microscopy.

Microscopy--Methodology.

Microscopy.

fluorescence microscopy.

Physical Description:

1 online resource (xviii, 178 pages) : illustrations, charts.

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume provides researchers with a comprehensive guide for understanding light-sheet fluorescence microscopy (LSFM), and covering issues in both its routine use of the method and the latest advancements. The chapters in this book are organized into three parts: Part One is an introduction to the method, its history, and a review on tissue clearing. Part Two focuses on recent advancements in LSFM that cover increasing resolution, field of view, and image contrast. Part Three presents several guides to the use of LSFM in an imaging facility, human brain imaging, mapping of immediate early genes, and in vivo functional imaging on Zebrafish. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Light-Sheet Microscopy is a valuable resource for all researchers who are interested in learning more about the basic and advanced uses of LSFM.

Contents:

The development of ultramicroscopy / Hans‑Urich Dodt

Tissue clearing / Fumito Akiyama, Katsuhiko Matsumoto, and Hiroki R. Ueda

Advanced tissue clearing and axially swept light-sheet microscopy for high-resolution imaging of neural systems / Hazel M. Borges, John Haug, Jinlong Lin, Samantha Calvache, Felix Y. Zhou, Xuemei Luo, Iliodora V. Pop, Zhenyu Zhong, Shuang Liang, Helen C. Lai, and Kevin M. Dean

Light-sheet fluorescence microscopy of expanded neuronal tissue / Juan Eduardo Rodriguez‑Gatica, Martin Karl Schwarz, and Ulrich Kubitscheck

Expansion lattice light-sheet microscopy reveals subcellular organization and molecular profile of the brain with nanoscopic resolution / Wei Wang and Ruixuan Gao

A guide to leveraging electronics metrology technologies for new light-sheet microscopy systems / Adam Glaser

RAPID autofocusing in light-sheet microscopy: an implementation guide / Ludovico Silvestri

Light-sheet in a multi-user facility / Laura Batti and Stéphane Pagès

Cell-resolution human brain reconstruction with light-sheet fluorescence microscopy / Irene Costantini, Luca Pesce, and Francesco Saverio Pavone

BRANT pipeline to map neuronal activations across the entire brain: an implementation guide / Alessandra Franceschini

High spatiotemporal resolution functional imaging of the entire larval zebrafish brain with two-photon light-sheet fluorescence microscopy / Lapo Turrini, Giuseppe de Vito, and Francesco Saverio Pavone.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed January 13, 2026).

Other Format:

Print version: Light sheet microscopy

ISBN:

9781071651001

1071651005

OCLC:

1565879899

Access Restriction:

Restricted for use by site license