Whole genome amplification : methods and protocols / edited by Yafeng Ma.

Format:

Book

Contributor:

Ma, Yafeng, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 3003.

Methods in molecular biology, 1940-6029 ; 3003

Language:

English

Subjects (All):

Gene amplification--Laboratory manuals.

Gene amplification.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource (xi, 226 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume focuses on the broad application of whole genome amplification (WGA) in cancers, preimplantation genetic testing, and forensic science. The chapters in this book cover topics such as upstream challenges (sample preparation, quality control) and downstream applications (next-generation sequencing, SNP detection). This book also explores how WGA’s have impacted fields like cancer genomics (detecting subclonal mutations), prenatal testing (non-invasive aneuploidy screening), and microbiome research (amplifying low-biomass communities). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Whole Genome Amplification: Methods and Protocols is a valuable resource for all researchers who are looking to learn more about this important and advancing field. Chapter 13 is available Open Access under a Creative Commons Attribution 4.0. International License via link.springer.com. .

Contents:

Whole-Genome Amplification for Circulating Tumor Cells

Whole Genome Amplification on Single Circulating Trophoblast Cell

A Tailing Method of Quality Control on MALBAC-Based Whole-Genome Amplification Products

Micromanipulation and Multiple-Strand Displacement Amplification for Forensic DNA Profiling of Single Sperm Cells

Whole-Genome Amplification of Single Circulating Tumor Cells from Mice Xenografts

An Integrated Preimplantation Genetic Testing Approach with MALBAC-Based Whole-Genome Sequencing

Handmade Biopsies and Whole Genome Amplification for Genotyping and Genomic Selection of Bovine Preimplantation Embryos

Accelerating Genomics: Innovations in Analyzing Limited and Rare Samples

Sweet Sorghum Microspores’ Fractionalization, Individual Isolation, and Whole Genome Amplification

Assessing Amplification Quality and Bias in MDA Methods through Comparative Analysis of Short-Read Sequencing

Sequencing RSV Whole Genome Using a Long Amplicon-Based Method with Oxford Nanopore Technologies

Laser Microdissection and Near Single-Cell Whole Genome Amplification

Whole Genome Amplification of Microbial DNA from Host-Depleted Clinical Samples

Whole Genomic Amplification for Detecting Single-Gene Variants Causing Inherited Disease

Single-Cell Low-Pass Whole Genome Sequencing of Circulating Tumor Cells

Single Oocyst Selection and Whole Genome Amplification of Cryptosporidium parvum.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed January 12, 2026).

Other Format:

Print version: Whole genome amplification

ISBN:

9781071650608

1071650602

OCLC:

1568100919

Access Restriction:

Restricted for use by site license