Introduction to Pharmaceutical Biotechnology. Volume 3, Animal Tissue Culture and Biopharmaceuticals / edited by Saurabh Bhatia and Ahmed Al-Harrasi.

Format:

Book

Contributor:

Bhatia, Saurabh, editor.

Al-Harrasi, Ahmed, editor.

Series:

IOP Ebooks Series

Language:

English

Subjects (All):

Pharmaceutical biotechnology.

Pharmaceutical technology.

Physical Description:

1 online resource (360 pages)

Edition:

Second edition.

Place of Publication:

Bristol, England : IOP Publishing, [2024]

Summary:

This third volume of the Second Edition of "Introduction to Pharmaceutical Biotechnology", focuses on animal tissue culture, with strong presentations of stem cell culture and engineering, with a special emphasis on their clinical use and applications.

Contents:

Intro

Acknowledgements

Editor biographies

Professor Ahmed Al-Harrasi

Dr Saurabh Bhatia

List of contributors

Chapter Introduction to animal tissue culture science

1.1 Introduction

1.2 Historical background

1.3 Types of cell cultures

1.4 Primary cell culture

1.4.1 Mechanical disaggregation

1.4.2 Enzymatic disaggregation

1.4.3 Primary explant technique

1.4.4 Organ culture

1.4.5 Non-enzymatic disaggregation

1.5 Segregation of non-viable cells from viable cells

1.6 Ethical issues in animal tissue culture

1.7 Safety considerations in animal tissue culture

1.7.1 Biosafety protocols for animal cell culture facilities

1.8 Cell lines (first subculture or passage)

1.8.1 Types of cell lines

1.8.2 Standard nomenclature of cell lines

1.8.3 Cell line selection

1.8.4 Verification of a cell line

1.8.5 Characterization of cell lines

1.8.6 Maintenance of a cell line

1.9 Subculture

1.9.1 Monolayer cultures

1.9.2 Procedures for cell detachment

1.10 Suspension cultures

1.10.1 Cell synchronization

1.10.2 Cell synchronization by chemical means

1.10.3 Cell synchronization by physical means

1.11 Algal extracts in animal tissue culture

1.12 Animal and plant tissue culture

1.13 Biomaterials and animal tissue culture

1.14 Nanotechnology and biotechnology

References

Chapter Organ culture

2.1 Organ culture

2.1.1 Organ culture techniques

2.1.2 Plasma clot

2.1.3 Raft methods

2.1.4 Grid method

2.1.5 Agar gel

2.1.6 Cyclic exposure to the medium and gas phase

2.2 Histotypic culture

2.2.1 Gel and sponge technique

2.2.2 Hollow fiber technique

2.2.3 Spheroids

2.2.4 Rotating chambers

2.3 Artificial skin

2.4 Ex vivo organ culture

2.4.1 Other ex vivo organ cultures

Chapter Stem cell culture.

3.1 Introduction

3.2 Embryonic stem cells

3.3 Epithelial stem cells

3.3.1 Stratified epithelial cell culture

3.3.2 Columnar epithelial cell culture

3.4 Cancer cell culture

3.5 Maintenance of stem cells

3.6 Adult stem cells

3.6.1 Mesenchymal stem cells

3.6.2 Mesenchymal stem cell culture technique based on microfluidic hanging-drop method

3.6.3 Application of MSCs in cancer treatment

3.7 Applications of cultured stem cells

3.7.1 Stem cell applications in regenerative medicine and disease therapeutics

3.7.2 Other applications

3.8 Stem cell therapy for SARS-CoV-2 virus

3.8.1 Challenges concerning stem cell treatment in Covid-19 patients

3.9 Recent progress in three-dimensional stem cell culture methodologies

3.9.1 Hydrogel-based stem cell culture technology

3.9.2 Liquid overlay culture

3.9.3 Rotating bioreactor culture

Chapter Culture media for animal cells

4.1 Introduction

4.1.1 Natural media

4.1.2 Artificial media

4.2 Physical and chemical properties of culture media

4.2.1 pH

4.2.2 Oxygen

4.2.3 Temperature

4.2.4 Osmolality

4.3 Balanced salt solutions (BSS)

4.4 Growth medium

4.4.1 The role of amino acids in animal culture

4.4.2 Vitamins

4.4.3 Micronutrients

4.4.4 Glucose

4.4.5 Hormones and growth factors

4.4.6 Additional organic supplements

4.5 Serum

4.5.1 Proteins

4.5.2 Nutrients and metabolites

4.5.3 Growth factors

4.5.4 Hormones

4.5.5 Carrier proteins

4.5.6 Lipids and related components

4.5.7 Transition metal

4.5.8 Vitamins

4.5.9 Polyamines

4.5.10 Reducing agents

4.5.11 Additives

4.5.12 Adhesion factors

4.6 Bioreactors in animal tissue culture

4.7 Serum-free media

4.7.1 Preparation of serum-free media

Chapter Animal tissue culture facilities

5.1 Introduction.

5.2 Infrastructural requirements

5.3 Equipment

5.3.1 Laminar flow hood

5.3.2 Incubator

5.3.3 Inverted microscope

5.3.4 Centrifuge

5.3.5 Water bath

5.3.6 Refrigerator and freezer

5.3.7 Osmometer

5.4 Supplementary equipment for tissue culture laboratory

5.4.1 Computers and networks

5.4.2 Upright microscope

5.5 Specialized equipment

5.5.1 Colony counter

5.5.2 Flow cytometer

5.6 Culture vessels

5.6.1 Materials used for culture vessels

5.6.2 Types of culture vessel

5.6.3 Cell culture vessel treatment

5.6.4 Non-adhesive substrates

5.7 Sterilization procedures in animal tissue cultures

5.7.1 Biological contamination

5.7.2 Chemical contamination

5.8 Aseptic conditions

5.9 Sterilization procedures

5.9.1 Dry heat sterilization/depyrogenation

5.9.2 Chemical methods of sterilization

5.9.3 Filtration

5.9.4 Moist heat sterilization

5.9.5 Gas sterilization

5.9.6 Solvents

5.9.7 Flaming sterilization

5.9.8 Sterile filtration

5.10 Advantages and disadvantages of animal tissue culture

5.11 Safety regulations for animal tissue culture laboratories

5.11.1 Cell cultures handling

5.11.2 Biohazards and safety concerns

5.11.3 Aerosol reduction and aseptic technology

5.11.4 Biohazards

Chapter Characterization of cultured cells

6.1 Introduction

6.1.1 Calcium-dependent proteins (cadherins)

6.1.2 Calcium-independent proteins

6.1.3 Proteoglycans

6.2 Cell proliferation, differentiation, and metabolism

6.3 Characterization of cultured cells

6.3.1 Recent advancements in characterization of cultured cells

6.3.2 Cell line misidentification

6.4 Parameters of characterization

6.4.1 DNA profiling or analysis of gene expression

6.4.2 Fluorescence in situ hybridization (FISH) or chromosome painting.

6.4.3 Major histocompatibility complex (MHC) analysis

6.4.4 Lineage or tissue markers

6.4.5 Unique markers

6.4.6 Transformation

6.5 Characterization of stem cells

6.5.1 Comparative genomic hybridization

6.5.2 Epigenetic profiling

6.5.3 Polymerase chain reaction (PCR)

6.5.4 Fluorescence in situ hybridization (FISH)

6.5.5 Karyotyping

6.5.6 Single nucleotide polymorphism (SNP) analysis

6.5.7 Pluripotency markers (proteins)

6.5.8 Western blot

6.5.9 RT-qPCR

6.5.10 Stem cell arrays

6.5.11 Flow cytometry

6.5.12 DNA extraction methods

6.5.13 Short tandem repeat (STR) analysis

6.6 Applications of animal cell cultures

6.6.1 Biopharmaceuticals from animal tissue culture

6.6.2 High value proteins

6.6.3 Cancer research

6.6.4 Model system

6.6.5 Gene therapy

6.6.6 Co-culturing of mammalian cells and algae

Chapter Role of pharmacists in delivering biotechnological products

7.1 Introduction

7.2 The role of the pharmacist

7.3 The route of administration for biopharmaceuticals

7.4 Development of biopharmaceuticals

7.5 Basic requirements for biotechnology-based products

7.6 Strategies adopted by pharmacists for dealing with biopharmaceuticals

7.6.1 Utilization of hematopoietic growth factors

7.6.2 Training for intramuscular administration

7.6.3 Pegnology and biopharmaceuticals

7.6.4 Biopharmaceutical distribution and preparation

7.6.5 Distribution

7.6.6 Excretion

7.6.7 Metabolism/catabolism

7.6.8 Anti-drug antibodies

7.6.9 Glycosylation

7.6.10 Toxicology

7.7 Protein crystals for the delivery of biopharmaceuticals

7.8 Patient care, education, training, and follow-up

7.9 Physical factor assessment (related to the serious medical history of the patient)

7.10 Emotional and environmental factor assessment.

7.11 Knowledge of products and suitable delivery systems

7.12 Preservatives and biopharmaceuticals

7.13 Dosing schedules

7.14 Education regarding adverse effects and prevention strategies for most biopharmaceuticals

7.15 Treatment with adjuvant

7.16 Dosage errors

7.17 Proper supply

7.18 Familiarity with terminology and the biotechnological literature

7.19 Dealing with expensive biopharmaceuticals

7.20 Generic biopharmaceuticals

7.21 Collaboration between pharmacies, wholesalers, and manufacturers

7.22 Reimbursement issues and biopharmaceuticals

7.23 Third party reimbursement issues with biopharmaceuticals

7.24 Affordable therapy and biopharmaceuticals

7.25 Information on the right reimbursement policy for biopharmaceuticals

7.26 Direct reimbursement of biopharmaceuticals

7.27 Dispensing fee reimbursement when dealing with biopharmaceuticals

7.28 Proper refrigeration of biopharmaceuticals

7.29 Stock management of biopharmaceuticals

7.30 Drug loss due to premature discontinuation of medications

7.31 The future pharmacist who can efficiently deal with biopharmaceuticals

7.32 Stability issues of biopharmaceuticals

References.

Notes:

Includes bibliographical references.

Description based on publisher supplied metadata and other sources.

Description based on print version record.

Other Format:

Print version: Bhatia, Saurabh Introduction to Pharmaceutical Biotechnology, Volume 3 (Second Edition)

ISBN:

9780750353946

OCLC:

1453194602