Molecular and functional characterisation of the novel nuclear basket protein ZC3HC1 / Philip Gunkel.

Format:

Book

Author/Creator:

Gunkel, Philip, author.

Language:

English

Subjects (All):

Molecular astrophysics.

Physical Description:

1 online resource (0 pages)

Edition:

First edition.

Place of Publication:

Gottingen : Cuvillier Verlag, [2023]

Summary:

The nuclear basket (NB) is a fibrillar structure anchored to the nuclear pore complex. The NB scaffold is formed by the coiled-coil dominated protein TPR. Other NB-resident proteins use the NB only as a binding platform without acting as a structural element themselves. We found that ZC3HC1 is a novel, bona fide component of the NB, which is required for the recruitment of TPR subpopulations to the NB, where ZC3HC1 acts as a linker to establish stable interconnections between TPR polypeptides positioned at the NB and to maintain the integrity of these assemblies. Consequently, ZC3HC1 allows for a higher complexity of the NB than previously anticipated. In addition, we characterised the molecular properties of ZC3HC1 that are prerequisites for its interaction with the NB and TPR polypeptides. We further revealed the evolutionary conservation of the TPR:ZC3HC1 interaction, exemplified by demonstrating that Pml39p is the structural and functional homologue of ZC3HC1 in Saccharomyces cerevisiae.

Contents:

Intro

Summary

List of Figures

List of abbreviations

Author contributionss

Chapter 1: Introduction and overview

1.1 The nuclear pore complex and the nuclear basket

1.2 NB scaffold protein TPR and its known interactors

1.3 Protein ZC3HC1 and its reported functions

1.4 Starting point for this project

1.5 ZC3HC1 is an NB protein in a state of reciprocal dependence with TPR

1.6 ZC3HC1 is the second structural element of the NB

1.7 Pml39p is the evolutionary conserved homologue of ZC3HC1 in budding yeast

Chapter 2: ZC3HC1 is a novel inherent component of the nuclear basket, resident in a state of reciprocal dependence with TPR

Chapter 3: ZC3HC1 is a structural element of the nuclear basket effecting interlinkage of TPR polypeptides

Chapter 4: Nuclear basket protein ZC3HC1 and its yeast homolog Pml39p feature an evolutionary conserved bimodular construction essential for initial binding to NPC-anchored homologs of scaffold protein TPR

Chapter 5: Discussion

References

Acknowledgements.

Notes:

Includes bibliographical references.

Description based on publisher supplied metadata and other sources.

Description based on print version record.

Other Format:

Print version: Gunkel, Philip Molecular and Functional Characterisation of the Novel Nuclear Basket Protein ZC3HC1

ISBN:

9783736968417