Duchenne muscular dystrophy : methods and protocols / edited by Kevin Flanigan and Stefan Nicolau.

Format:

Book

Contributor:

Flanigan, Kevin, editor.

Nicolau, Stefan, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2975.

Methods in molecular biology, 1940-6029 ; 2975

Language:

English

Subjects (All):

Duchenne muscular dystrophy--Laboratory manuals.

Duchenne muscular dystrophy.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (xiii, 298 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease. .

Contents:

Multiplex ligation-dependent probe amplification (MLPA) for the detection of copy number mutations in the DMD gene / Diane M. Dunn & Robert B. Weiss

Total RNA-seq as a tool to study DMD splicing and transcriptional dynamics / Diane M. Dunn & Robert B. Weiss

Preparation, sectioning, and histochemical staining of muscle tissue / Emma C. Frair & Stefan Nicolau

Manual immunofluorescence staining for dystrophin and key dystrophin-associated proteins / Hui Meng, Emily M. Ott, Mariah J. Prom, Margaret J. Beatka, Tatyana A. Vetter & Michael W. Lawlor

Automated quantification of dystrophin expression by immunofluorescence in humans and animal models / Tatyana A. Vetter & Michael W. Lawlor

Quantitative evaluation of dystrophin expression using SDS-PAGE western blot methods / Margaret J. Beatka, Mariah J. Prom, Margaret Haberman, Jessica Sutton, J. Patrick Gonzalez & Michael W. Lawlor

Analysis of dystrophin-associated glycoproteins : Focus on expression and glycosylation of dystroglycan in skeletal muscle / Deborah Zygmunt, Patricia Lam & Paul T. Martin

Quantification of dystrophin expression by capillary immunoassay / Stefan Nicolau

Quantification of dystrophin in human muscle biopsies by mass spectrometry / Emily H. Canessa & Yetrib Hathout

Isolation and culture of primary myoblasts from humans and mice / Felipe de Souza Leite & Emanuela Gussoni

Isolation of immune cells from skeletal muscles for flow cytometry / Zachary M. Howard, Jeovanna Lowe & Jill A. Rafael-Fortney

Exploring therapies for Duchenne muscular dystrophy using transdifferentiated patient fibroblasts / Camila F. Almeida & Nicolas Wein

Culture and electrophysiological analysis of patient-specific iPSCs using microelectrode array dishes / Bayardo I. Garay & Rita C. R. Perlingeiro

Canine model of Duchenne muscular dystrophy / James Teixeira, Matthew J. Burke, Roland W. Herzog & Dongsheng Duan

In vivo electroporation of plasmid DNA into the skeletal muscle of dystrophic mouse models / Miguel A. Lopez Perez, Gianni N. Giarrano & Noah L. Weisleder

Intramuscular and intravenous AAV-mediated gene delivery in mouse models / Liubov V. Gushchina

Assessment of contractile and kinetic properties of skeletal and cardiac multicellular preparations in mouse models : A comprehensive methodological guide / Mohammed A. Mashali, Nancy S. Saad, Neha Rastogi & Paul M. L. Janssen.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 16, 2025).

Other Format:

Print version: Duchenne muscular dystrophy

ISBN:

9781071648117

107164811X

OCLC:

1545533303

Access Restriction:

Restricted for use by site license