Digital PCR : methods and protocols / edited by Guillaume Pavlovic.

Format:

Book

Contributor:

Pavlovic, Guillaume, 1976- editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 2969.

Methods in molecular biology, 1940-6029 ; 2969

Language:

English

Subjects (All):

Polymerase chain reaction--Technological innovations--Laboratory manuals.

Polymerase chain reaction.

Polymerase Chain Reaction.

Medical Subjects:

Polymerase Chain Reaction.

Physical Description:

1 online resource (xi, 288 pages) : illustrations (som color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Digital PCR: Methods and Protocols serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision"-- Springer Nature Link.

Contents:

A beginner step-by-step guide for gene expression analysis using QIAcuity digital PCR / Jonathan Weiss, Gerald Schock, Afif Abdel Nour, Katharina Schaepe, Kevin Matthaei, and Ralf Peist

Multiplex quantification of 19 GM soybean lines using digital PCR / Amadej Jelenc?ic´, Dejan Štebih, Tina Demšar, and David Dobnik

Multiplex digital PCR assay targeting DNA methylation for early detection of cancer / Victoria Cannon, Jocelyn H. Wright, Cecilia C. S. Yeung, William M. Grady, and Ming Yu

Multiplexed digital PCR for evaluation of karyotype quality of mouse embryonic stem cells / Christy J. Greenwood, Alasdair J. Allan, and Lydia Teboul

Optimizing digital PCR assays for multiplexed microbial source tracking in surface waters / Shimul Ghosh and Aaron Bivins

Crystal Digital PCR™-based single nucleus pollen genotyping in barley (Hordeum vulgare L.) to measure meiotic crossover rates in defined chromosomal intervals / Suriya Tamilselvan-Nattar-Amutha, Jörg Fuchs, and Stefan Heckmann

Robust dPCR hyper-plexing approach by color combination for detection of 17 ESR1 mutations in breast cancer patient samples / Sylvain Ursuegui, Julie Donzier, Cécile Jovelet, Etienne Fradet, and Rémi Dangla

Digital PCR analysis of methylated circulating tumor DNA / Rikke Fredslund Andersen, Tina Brandt Christensen, and Torben Frøstrup Hansen

Method for validation multiplexed droplet digital PCR for the detection of copy number variants in cultured human pluripotent stem cells / Said Assou, Reda Zenagui, Juline Vincent, Laurent Manchon, and John De Vos

Digital PCR methods for discovering molecular markers for Hanwoo cattle breed genotyping / Songmi Kim, Jinuk Jeong, Seyoung Mun, and Kyudong Han

A duplex digital PCR method for quantification of transgene soybean line MON40-3-2 in complex matrices / Alexandra Bogožalec Kos?ir, Tina Demšar, Dejan Štebih, and Mojca Milavec

DNA methylation analyses of tissue samples and cell-free DNA by droplet digital PCR / Heidi Pharo, Sara Brandt-Winge, Evy Marie Thorkildsen, Hege Marie Vedeld, and Guro E. Lind

Quantification of tissue and circulating microRNAs by droplet digital PCR / Elisabetta Broseghini, Beatrice Fontana, Giorgio Durante, Roberta Roncarati, and Manuela Ferracin

Digital droplet PCR (ddPCR) for absolute quantification of 16S rRNA copy number in metagenomic data / Claudia Leoni, Marinella Marzano, Ermes Filomena, and Anna Maria D’Erchia

Extending the potential of digital droplet PCR by expressing amplified genes and assaying their phenotype / Janine Kehrli, Radi Khodr, and Michael Ryckelynck

20-plex gene expression analysis using hyperplex PCR (hpPCR) toward the development of salt-tolerant wheat / Esteri Viitanen, Samuel Narmack, Margarita Psallida, Johan Banér, Umear Naseem, Ruben R. G. Soares, and Henrik Aronsson.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 28, 2025).

Other Format:

Print version: Digital PCR

ISBN:

9781071647677

1071647679

OCLC:

1547386743

Publisher Number:

CIPO000286392

CIPO000286391

Access Restriction:

Restricted for use by site license