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Autoimmunity : methods and protocols / edited by Rami Bechara
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; 1064-3745 v. 2990.
- Methods in molecular biology, 1940-6029 ; 2990
- Language:
- English
- Subjects (All):
- Autoimmune diseases--Laboratory manuals.
- Autoimmune diseases.
- Autoimmunity--Laboratory manuals.
- Autoimmunity.
- Medical Subjects:
- Autoimmunity.
- Genre:
- Laboratory manuals
- Laboratory manuals.
- Physical Description:
- 1 online resource (xii, 284 pages) : illustrations (chiefly color)
- Place of Publication:
- New York, NY : Humana Press, [2026]
- Summary:
- This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
- Contents:
- Identification and characterization of human autoreactive T cells for autoimmune research / Lenka Súkeníková and Daniela Latorre
- T cell antigen discovery using cell-based epitope libraries / Paul M. Zdinak, Sanya Arshad, Venkata Krishna Kanth Makani, and Alok V. Joglekar
- Purification, phenotypic and functional analyses of regulatory T cells in autoimmunity / Nainisha Shah, Annemarie Fortuin, Georgia Afonso, and Pascale Louis-Plence
- Culturing murine bone marrow-derived macrophages / Becky Lin and Giraldina Trevejo-Nuñez
- Macrophage cell culture and characterization by serum human AB in health and disease / Audrey Paoletti, Bineta Ly, Samuel Bitoun, Gaetane Nocturne, and Xavier Mariette
- Isolation and culture of rheumatoid arthritis synoviocytes / Mélissa Noack
- Comprehensive assessment of neutrophil function in autoimmune diseases / Quiterie Labaye, Daouda Abba Moussa, and Mar Naranjo-Gomez
- Neutrophil transmigration analyses for multiplexed drug screening and in vivo-like application testing using an organ-on-a-chip model / Clara M. Reichardt and Luis E. Muñoz
- Induction, isolation, and characterization of human neutrophil extracellular traps (NETs) to be used in functional assays / Quang Khai Doan, Cindy Qiu, and Patrice Decker
- Analysis of cellular metabolism using flow cytometry / Christos Aronis and Matteo Villa
- Quantifying mitochondrial metabolism and metabolic fluxes in soft agar cultures / Birte Dowerg, Fangfang Chen, and Thekla Cordes
- Assessing mitochondrial respiration of in vitro models for the study of immune-related diseases using a Seahorse analyzer / Chloé Chivé and Arnaud Tête
- Investigating dysregulated X chromosome inactivation in human B cells and plasmacytoid dendritic cells by RNA FISH / Berenice Faz-Lopez, Sophie Allart, Julie Chaumeil, and Jean-Charles Guéry
- Single-cell spatial transcriptomic of Sjögren’s disease salivary glands / Charlotte Gandubert, Simon Höllerer, Lisa Sériau, Soizic Garaud, Christelle Le Dantec, Valérie Devauchelle-Pensec, Sophie Hillion, Divi Cornec, and Marie Frutoso
- Establishment of adult stem cell-derived organoid cultures : General principles from the mouse gut / Kadi Lõhmussaar
- In vitro lymphoid differentiation of human hematopoietic progenitor cells from co-cultures in hypoxia / Françoise Pflumio and Rima Haddad
- Analysis of microRNAs expression across cells, tissues, and body fluids / Julie Perrot, Imene Hani, Laetitia Martinerie, and Say Viengchareun
- Polysome profiling : A method for global and targeted analysis of mRNA translation / Lucilla Fabbri, Ana Luisa Dian, Céline M. Labbé, and Stéphan Vagner
- A versatile ribo-seq workflow for enhanced analysis of ribosome dynamics / Hélène Hérault, Armelle Choquet, and Alexandre David.
- Notes:
- Includes bibliographical references and index
- Online resource; title from PDF title page (SpringerLink, viewed December 23, 2025).
- Other Format:
- Print version: Autoimmunity
- ISBN:
- 9781071649978
- 1071649973
- OCLC:
- 1558487849
- Publisher Number:
- CIPO000323652
- Access Restriction:
- Restricted for use by site license
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