Plant biotic stress : methods and protocols / edited by Muthappa Senthil-Kumar, Hema Ramanna.

Format:

Book

Contributor:

Senthil-Kumar, Muthappa, editor.

Ramanna, Hema, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2966.

Methods in molecular biology, 1940-6029 ; 2966

Language:

English

Subjects (All):

Plants--Effect of stress on--Research--Methodology.

Plants.

Plants--Effect of stress on.

Physical Description:

1 online resource (xii, 317 pages) : illustrations.

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant-pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Contents:

Plant biotic stress : Tools and techniques for crop protection / Krishna Ramakrishnan Acharya, Aswin Reddy Chilakala, and Muthappa Senthil-Kumar

Investigating the involvement of target protein in plant‑pathogen interactions through ectopic expression in Arabidopsis / Annapurna Sahoo, Sanjoy Majumder, Rutupurna Das, Kunja Bihari Satapathy, and Gagan Kumar Panigrahi

RNA interference strategy for the development of okra (Abelmoschus esculentus L. Moench) lines resistant to yellow vein mosaic virus / Vipul Ganesh Kelkar, Deepu Mathew, Shivaji Ajinath Lavale, and Feba Jacob

Spot inoculation of begomovirus by single whitefly (Bemisia tabaci) transmission in mungbean using microcage / Shikha Dixit, Pandian Rajendran, Revanasiddappa, Bhimeshwari Sahu, J. Sridhar, Ashish Marathe, and Palaiyur Nanjappan Sivalingam

One‑step RT‑LAMP : A method for closed‑tube colorimetric detection of RNA viruses in plants / Smita Nair and K. Midhuna Madhu

Detection of sesame phyllody disease and its transmitting vectors / K. B. Durga Bhavani, E. Ashwini, V. Dinesh Kumar, T. Boopathi, and P. Duraimurugan

A method for isolation and transfection of Arabidopsis protoplast for sucrose feeding assay / Anjali Anjali and Muthappa Senthil-Kumar

Twitching motility dynamics in the wilt‑causing phytopathogen Ralstonia solanacearum / Shuvam Bhuyan, Lukapriya Dutta, Shuhada Begum, Shubhra Jyoti Giri, Monika Jain, Manabendra Mandal, and Suvendra Kumar Ray

A blotting paper technique for the screening of chickpea genotypes against dry root rot disease / Athimoolam Durgadevi, Prachi Pandey, and Muthappa Senthil-Kumar

CRISPR‑mediated gene editing for inducing thermosensitive genic male sterility and sheath blight resistance in rice / Shanthinie Ashokkumar, Vignesh Ponnurangan, Kumar K. Krish, Arul Loganathan, Kokiladevi Eswaran, Paranidharan Vaikuntavasan, Sudhakar Duraialagaraja, and Varanavasiappan Shanmugam

A seedling root dip‑based technique to screen chickpea for resistance to Fusarium wilt disease / B. S. Patil, Vijaykumar K. N, K. S. Iliger, C. Bharadwaj, and Shailesh Tripathi

Oligo‑dT anchored cDNA SCoT : An innovative approach for deciphering Fusarium wilt resistance‑related differential gene expression in chickpea / S. L. Abhang, P. Sharma, S. N. Datarkar, M. P. Moharil, H. Kudapa, A. P. Ingle, and P. V. Jadhav

Extracellular acidification assay to evaluate the effectiveness of antifungal agents on the pathogenicity of Macrophomina phaseolina / Krishna Ramakrishnan Acharya, Aswin Reddy Chilakala, and Muthappa Senthil-Kumar

A guide to culturing, maintenance, and leaf inoculation methods for rapid screening and quantification of Sclerotinia sclerotiorum infection in mustard / Bhanu Malhotra, Ruchi Tiwari, Mohan Varghese, and Naveen C. Bisht

Differential‑display reverse transcription‑PCR for unraveling the molecular mechanisms in foot rot (Phytophthora capsici sp. nov.) tolerance in black pepper (Piper nigrum L.) / Basil Babu Paul and Deepu Mathew

Estimation of foliar volatiles emitted by mycorrhizal‑colonized blackgram (Vigna mungo L.) infested with Spodoptera litura / Selvaraj Anandakumar, Dhandapani Senthamilselvi, and Thangavel Kalaiselvi

Development of an Agrobacterium tumefaciens‑mediated in planta transformation strategy in pigeonpea for resistance to Helicoverpa armigera / Maniraj Rathinam and Rohini Sreevathsa

CRISPR/Cas9‑mediated genome editing in the management of oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae : Diptera) / Chikmagalur Nagaraja Bhargava, Ashok Karuppannasamy, and Asokan Ramasamy

A novel approach using SSR markers for identifying and breeding bruchid pest‑resistant blackgram (Vigna mungo L. Hepper) genotypes / Ragul Subramaniyan, Manivannan Narayana, and Soundararajan Raga Palanisamy

RNAi‑based approach as a promising tool for management of leafhopper (Amrasca biguttula biguttula) in cotton / K. Shankarganesh, V. Gowtham, K. P. Raghavendra, and Joy Das

Germplasm evaluation in chickpea for resistance to plant parasitic nematodes / Zakaullah Khan, Bharat H. Gawade, Gayacharan, and V. Celia Chalam

Novel method for rapid screening of chickpea for combined dry root rot disease and osmotic stress / Shubhashish Ranjan, Chaitali Narendra Chavan, and Muthappa Senthil-Kumar.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 14, 2025).

Other Format:

Print version: Plant biotic stress

ISBN:

9781071647462

1071647466

OCLC:

1545097574

Publisher Number:

CIPO000286386

CIPO000286387

Access Restriction:

Restricted for use by site license