Cryptosporidium : methods and protocols / edited by Jan R. Mead, Michael J. Arrowood.

Format:

Book

Contributor:

Mead, Jan R., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology, 1940-6029 ; 2978

Language:

English

Subjects (All):

Cryptosporidium--Laboratory manuals.

Cryptosporidium.

Cryptosporidiosis--Laboratory manuals.

Cryptosporidiosis.

Cryptosporidium--Research.

Genre:

Laboratory manuals

Physical Description:

1 online resource (xv, 405 pages) : illustrations (some color).

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This second edition volume expands on the previous edition with new chapters discussing the latest protocols that encompass the detection, characterization, and genomic analysis of Cryptosporidium. The first few chapters in this book focus on diagnostic assays, such as traditional microscopy-based techniques, nucleic acid-based molecular detection, and immunoassay-based methodologies. Several chapters cover whole genome sequencing methodologies, genotyping approaches, and novel techniques such as hybridization bait capture analyses. A significant portion of this book is dedicated to functional genomics and experimental models, with discussions on tools used for genetic manipulation (including CRISPR/cas9), gene silencing, and host-pathogen interactions. The volume concludes with chapters exploring drug discovery and testing, highlighting high-content screening methods, phenotypic assays, and efficacy testing of potential disinfectants and therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cryptosporidium: Methods and Protocols, Second Edition is a valuable tool for researchers interested in gaining more insight on foundational and innovative Cryptosporidium research, which will inspire future advancements in this field"-- Springer Mature.

Contents:

Cryptosporidium diagnostic assays : microscopy / Guy Robinson and Rachel M. Chalmers

Cryptosporidium diagnostic assays : molecular detection and species determination by real-time PCR-based assays / Guy Robinson, Kristin Elwin, and Rachel M. Chalmers

Detection of Cryptosporidium recovered from large-volume water samples using dead-end ultrafiltration / Amy M. Kahler and Mia C. Mattioli

Genomic characterization of Cryptosporidium spp. via iNextEra library preparation and hybridization bait capture / Mohammad Imtiaj Uddin Bhuiyan, Fiifi Agyabeng-Dadzie, Amanda H. Sullivan, Megan S. Beaudry, Kelly N. Petersen, Piotr T. Tuczapski, Michael E. Grigg, Jessica C. Kissinger, and Travis C. Glenn

Preparation of Cryptosporidium for whole-genome sequencing / Guy Robinson and Rachel M. Chalmers

A real-time PCR-based method for the sequence determination of Cryptosporidium hominis and Cryptosporidium parvum gp60 subtypes during epidemiological investigations / Guy Robinson, Kristin Elwin, and Rachel M. Chalmers

Cryptosporidium genotyping for epidemiology tracking / Matthew H. Seabolt and Dawn M. Roellig

A multilocus variable-number tandem-repeat analysis (MLVA) scheme for epidemiological investigations of Cryptosporidium parvum / Harriet Risby, Guy Robinson, and Rachel M. Chalmers

Optimized genome assembly protocol for Cryptosporidium / Wanyi Huang and Lihua Xiao

Evolutionary genomic analyses of Cryptosporidium / Tianpeng Wang and Lihua Xiao

A novel method to silence genes in Cryptosporidium / Alejandro Castellanos-Gonzalez

Use of ncRNAs to study host cell–parasite interactions / Marion L. Graham and Xian-Ming Chen

Genetic manipulation of Cryptosporidium parvum / Sebastian Shaw, Adam Sateriale, Mattie C. Pawlowic, Sumiti Vinayak, Carrie F. Brooks, Jessica H. Byerly, and Boris Striepen

Mouse models for use in Cryptosporidium infection studies, quantification of parasite burden using flow cytometry, qPCR and histopathology, and confocal imaging of oocysts / Karine Sonzogni-Desautels, Jackson F. Chen, Jan R. Mead, and Momar Ndao

Calf clinical model of cryptosporidiosis for efficacy evaluation of therapeutics / Michael W. Riggs and Deborah A. Schaefer

Cell culture infectivity to assess chlorine disinfection of Cryptosporidium oocysts in water / Jennifer L. Murphy and Michael J. Arrowood

High-content screening for Cryptosporidium drug discovery / Malina A. Bakowski, Melissa S. Love, and Case W. McNamara

Phenotypic high-throughput screening and drug efficacy test against the growth of Cryptosporidium parvum in vitro by qRT-PCR / Chenchen Wang, Dongqiang Wang, Haili Zhang, and Guan Zhu

Establishing TurboID proximity labeling and mAID conditional knockdown system in Cryptosporidium parvum / Rui Xu and L. David Sibley

In vitro culture of Cryptosporidium spp. using stem cell-derived intestinal epithelial monolayers / Valentin Greigert, Georgia Wilke, Yi Wang, Soumya Ravindran, Matthew Freese, Thaddeus Stappenbeck, William Witola, and L. David Sibley

Two- and three-dimensional human intestinal tissue models for Cryptosporidium infection / Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, and Honorine D. Ward

Cryptosporidium oocyst purification using discontinuous gradient centrifugation / Michael J. Arrowood.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 19, 2025).

Other Format:

Print version: Cryptosporidium.

ISBN:

9781071648247

1071648241

OCLC:

1552172691

Publisher Number:

CIPO000294216

Access Restriction:

Restricted for use by site license