T. cruzi infection : methods and protocols / edited by Karina Andrea Gómez, Carlos Andrés Buscaglia.

Format:

Book

Contributor:

Gómez, Karina Andrea, editor.

Buscaglia, Carlos Andrés, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 2982.

Methods in molecular biology, 1940-6029 ; 2982

Language:

English

Subjects (All):

Trypanosoma cruzi--Laboratory manuals.

Trypanosoma cruzi.

Chagas' disease.

Chagas Disease.

Medical Subjects:

Chagas Disease.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource (xvi, 445 pages) : illustrations (chiefly color).

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This fully updated volume explores experimental methods to assess the complex interactions between Trypanosoma cruzi and its host and to study different aspects of T. cruzi and Chagas disease. The techniques presented, which touch on areas of bioinformatics, biochemistry, genetics, and cell biology research, have been recently developed or adapted to the T. cruzi system by leading experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, T. cruzi Infection: Methods and Protocols, Second Edition provides a comprehensive understanding of the basic biology of this parasite and its crosstalk with the host immune response that aims to inspire further understanding of Chagas disease epidemiology, diagnosis, treatment, and clinical evaluation.

Contents:

Computer-aided discovery of drugs against Chagas disease / Carolina L. Bellera, Lucas N. Alberca, and Alan Talevi

Chromatin interaction maps of Trypanosoma cruzi / Florencia Díaz‑Viraqué and Carlos Robello

GCanner, a genome-wide GC composition tool for the unbiased assessment of Trypanosoma cruzi genomic compartments / Virginia Balouz and Carlos Andrés Buscaglia

An algorithm for annotation of Trypanosoma cruzi MASP sequences / Aldana A. Cepeda Dean, Virginia Balouz, and Carlos Andrés Buscaglia

Cloning-free genome editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi / Miguel A. Chiurillo, Milad Ahmed, César González, Juliana Nunes Roson, Asima Das, and Noelia Lander

Species typing of trypanosomatids using amplicon-based sequencing / Cristian Canizales‑Silva, Luz Helena Patiño, and Juan David Ramírez

Deep sequencing of mHVRs for Trypanosoma cruzi genotyping / Anahí G. Díaz, Fanny Rusman, and Nicolás Tomasini

Identification of Trypanosoma cruzi discrete typing units by multiplex real-time PCR / Carolina Inés Cura and Juan Carlos Ramírez

Real-time PCR assays for quantification of Trypanosoma cruzi in food samples : A tool for surveillance of oral Chagas disease outbreaks / Otacilio C. Moreira, Amanda Faier‑Pereira, Carolina Valença‑Barbosa, and Paula Finamore‑Araujo

Ultra-rapid DNA extraction coupled with loop-mediated isothermal amplification for point-of-care detection of Trypanosoma cruzi infection in heparinized blood and dried blood spots / Silvia A. Longhi, Lady J. García‑Casares, Arturo A. Muñoz‑Calderon, Julio Alonso‑Padilla, and Alejandro G. Schijman

Quantitative real-time PCR for Trypanosoma cruzi infection diagnosis and treatment response monitoring of patients with Chagas disease / Juan Carlos Ramírez and Otacilio C. Moreira

Standardization of serodiagnostic methods for Trypanosoma cruzi / Fred Luciano Neves Santos

Software and tools to analyze high-density peptide array data for the Chagas antigen and epitope atlas / Guadalupe Romer, Ramiro B. Quinteros, and Fernán Agüero

Conjugation of saccharidic synthetic antigens with carrier proteins by the squaric acid method : Application as biomarkers for treatment evaluation in Chagas disease / M. Eugenia Giorgi, Linda Toro Melgarejo, Esteban Bodmer, and Carla Marino

Identifying Trypanosoma cruzi proteins targeted by Chagas disease patient antibodies using yeast surface display / Mira Loock and Igor Cestari

Profiling T cell receptor and immunoglobulin repertoires through RNA sequencing to understand host response to Trypanosoma cruzi infection / Rachel Clear, Claudia Herrera, and Eric Dumonteil

The use of B-cell tetramers for the study of Trypanosoma cruzi–specific B cells / Damián Pérez‑Mazliah

ELISPOT-based measurement of antibody-secreting cells and memory B cells specific to Trypanosoma cruzi in chronically infected subjects / Gonzalo Leandro Cesar, María Belén Caputo, Josefina Elias, María Gabriela Alvarez, Susana Adriana Laucella, and María Cecilia Albareda

Host-derived molecules as novel Chagas disease biomarkers : Hypercoagulability markers and extracellular vesicles / Berta Barnadas‑Carceller, Dolors Tàssies, Irene Losada Galván, Julio Alonso‑Padilla, Joan Carles Reverter, Carmen Fernandez‑Becerra, and María‑Jesús Pinazo

Advanced identification of Trypanosoma cruzi–specific CD4+ and CD8+ T cells in chronic Chagas disease patients using activation-induced marker (AIM) assay / Fátima Ferragut, Karen M. Cruz, and Karina Andrea Gomez

Assessing the metabolic profile of CD4+ and CD8+ T lymphocytes in chronic Chagas disease patients using SCENITH / Karen M. Cruz, Fátima Ferragut, Felipe Flores‑Santibañez, Rafael J. Argüello, and Karina Andrea Gomez

A single-cell methodology for energy metabolism analysis in parasitic protozoa / Salomé C. Vilchez‑Larrea, Rafael J. Argüello, and Guillermo D. Alonso

Determining histone posttranslational modifications along the cell cycle in Trypanosoma cruzi by flow cytometry / María del Rosario Lopez, Guillermo D. Alonso, and Josefina Ocampo

Ultrastructure expansion microscopy for the analysis of Trypanosoma cruzi infection / Felipe Castro Capurro and Victoria Lucia Alonso

An in vitro 3D collagen‑I model for studying Trypanosoma cruzi trypomastigote motility / Maximiliano Cosenza, Matías Exequiel Rodríguez, Ana González Wusener, Yamil E. Masip, and Valeria Tekiel

A rapid method for purifying intact Trypanosoma cruzi amastigotes from host cells, in vitro and in vivo, for downstream ’omic analysis / Francisco Olmo, Shiromani Jayawardhana, Richard L. Atherton, and John M. Kelly

Insights into the procedures and interpretation of autophagy in Trypanosoma cruzi / Gabriel Ferri, María Belén Lopez, Juan Agustín Cueto, María Cristina Vanrell, and Patricia Silvia Romano

Animal models for studying Trypanosoma cruzi congenital transmission and associated pathogenesis : Special emphasis on guinea pig model / Antonio Ortega‑Pacheco and Nisha J. Garg.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 25, 2025).

Other Format:

Print version: T. cruzi infection.

ISBN:

9781071648483

1071648489

OCLC:

1553741700

Publisher Number:

CIPO000320839

Access Restriction:

Restricted for use by site license