Lentiviral vectors : applications for basic and clinical neuroscience / edited by Kazuto Kobayashi, Hirokazu Hirai, Hiroyuki Hioki.

Format:

Book

Contributor:

Kobayashi, Kazuto, editor.

Hirai, Hirokazu, editor.

Hioki, Hiroyuki, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2974.

Methods in molecular biology, 1940-6029 ; 2974

Language:

English

Subjects (All):

Lentiviruses--Laboratory manuals.

Lentiviruses.

Neurosciences.

Virus-vector relationships.

Medical Subjects:

Neurosciences.

Physical Description:

1 online resource (xii, 270 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume provides readers with an overview of lentiviral vector applications and includes detailed laboratory protocols relevant to neuroscience research. The chapters are organized into five sections: Section I provides an overview of lentiviral vector properties, including practical methods for vector preparation and delivery into the brains of rodents and non-human primates, as well as delivery into non-human primate embryos for transgenic animal production. Sections II to IV explore basic neuroscience covering anatomical, molecular and developmental, and behavioral and physiological neuroscience. Section V focuses on clinical neuroscience, offering protocols related to gene therapy for motor neuron and ocular diseases. Written in the highly successful Methods in Molecular Biology series format, each chapter includes an introduction to the topic, a list of required materials and reagents, step-by-step protocols that are easy to reproduce, and expert tips for troubleshooting and avoiding common pitfalls. Cutting-edge and thorough, Lentiviral Vectors: Application for Basic and Clinical Neuroscience is a valuable resource for any researcher looking to learn more about this important and advancing field.

Contents:

Properties and application of pseudotyped lentiviral vectors / Natsuki Matsushita, Masahiko Takada, and Kazuto Kobayashi

Production and purification of lentiviral vectors / Kenta Kobayashi and Natsuki Matsushita

Lentiviral vector administration into the mouse brain / Hiromi Sano, Satomi Chiken, and Atsushi Nambu

Stereotaxic injection of lentiviral vectors into the primate brain / Ken-ichi Inoue and Masahiko Takada

Optimized lentiviral vector production using dual-pseudotyping with VSV-G and Sendai virus HN glycoproteins for enhanced gene delivery in diverse cell types / Bat-Erdene Jargalsaikhan, Masanaga Muto, and Masatsugu Ema

Preparation of lentiviral vectors for the generation of transgenic / Masanaga Muto and Masatsugu Ema

Ubiquitous and brain cell-type-specific promoters and enhancers in lentiviral vectors / Yasunori Matsuzaki, Ayumu Konno, and Hirokazu Hirai

Efficient labeling of somatodendritic membranes of the central neurons / Hiroshi Kameda, Shinichiro Okamoto, Masato Koike, Takahiro Furuta, and Hiroyuki Hioki

Visualization of selective neural pathways by double viral infection method / Akiya Watakabe

Lentivirus-mediated measurement of transcription factor activity in the brain / Kentaro Abe

Birth date-dependent tracing of neuronal cell lineage / Tatsumi Hirata

Application of lentivirus vectors to adult neurogenesis and manipulation of neural stem cells / Itaru Imayoshi, Mayumi Yamada, Shinji C. Nagasaki, Tomonori D. Fukuda, Ikumi Nagano, and Tomohisa Toda

Direct reprogramming of microglia into neurons / Kanae Matsuda-Ito, Eriko Hatai, Takashi Irie, Taito Matsuda, and Kinichi Nakashima

Selective neural pathway targeting by using lentiviral vectors / Shigeki Kato

Synaptic silencing with transactivation of tetanus neurotoxin for pathway-selective manipulation / Tadashi Isa, Kaoru Isa, and Wim Vanduffel

Optogenetic approach for in vivo manipulation of neural circuits using lentiviral vectors / Takashi Matsuda

Selective chemogenetic blockade of cerebello-rubral pathway by double-virus infection method using lentivirus and adeno-associated virus in rats / Hideki Hida and Shinya Ueno

Development of a gene therapy with lentiviral vectors for spinocerebellar ataxias / Tiago Moreira-Gomes and Clévio Nobrega

Lentiviral vector-mediated gene delivery for iPSC-related neuroscience research / Taro Okunomiya, Keiko Imamura, Taku Kashiyama, Takashi Sakurai, Hiroyuki Hioki, and Haruhisa Inoue

Phase 1/2a clinical trial protocol for lentiviral vector-based retinal gene therapy to slow the progression of retinitis pigmentosa / Takahiro Hisai, Yusuke Murakami, Kohji Kusano, Yuko Kobayakawa, and Yasuhiro Ikeda

CAR-T cell therapy for central nervous system tumors / Masasuke Ohno and Mitsugu Fujita.

Notes:

Includes bibliographical references at the end of each chapter and index.

Online resource; title from PDF title page (SpringerLink, viewed December 30, 2025).

Other Format:

Print version: Lentiviral vectors

ISBN:

9781071648070

1071648071

OCLC:

1565021489

Publisher Number:

CIPO000286403

CIPO000286402

Access Restriction:

Restricted for use by site license