Apomixis : methods and protocols / edited by Tatyana Radoeva and Peter J. van Dijk.

Format:

Book

Contributor:

Radoeva, Tatyana, editor.

Dijk, Peter van (Peter J.), editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in Molecular Biology, 1940-6029 ; 2987

Language:

English

Subjects (All):

Apomixis--Laboratory manuals.

Apomixis.

Genre:

Laboratory manuals

Physical Description:

1 online resource (xiv, 302 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume explores the latest research techniques (and reviews) used to study apomixis model species. The chapters in this book are organized into six parts: Part One introduces gametophytic apomixis, and Part Two focuses on the detection and characterization of apomixis. Part Three talks about transcriptomic profiling of apomictic and sexual species, and Part Four covers genetic analysis of apomixis. Part Five is dedicated to research on model apomictic systems such as dandelion (Taraxacum), Pilosella, Boechera, and Cenchrus (Pennisetum), and the final Part Six looks at protocols for engineering either individual components of apomixis, such as apomeiosis or a complete synthetic apomixis system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Apomixis: Methods and Protocols is a valuable reference for studying apomixis, and serves as an inspiration and foundation for designing new approaches and achieving significant breakthroughs in the field. Chapter 17 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Gametophytic apomixis : An introduction / Peter J. van Dijk and Tatyana Radoeva

Flow cytometric seed screening and seed genotyping : Methods for detecting reproductive modes in flowering plants / Petra Šarhanová, Luboš Majeský, and Juraj Paule

Analysis of gametophytic apomixis using confocal microscopy / Camilla Banfi and Philippa Jane Barrell

3D imaging, segmentation, and cell annotation of the ovule during megaspore mother cell differentiation in Paspalum spp. / Luciana Delgado and Daphné Autran

Imaging of callose in young ovules of diplosporous species of the Asteraceae family / Krystyna Musial and Agnieszka B. Springer

A method for detecting parthenogenesis in Asteraceae species / Tatyana Radoeva and Peter J. van Dijk

Laser-assisted microdissection for cell- and tissue type-specific transcriptome and RNA-seq analysis / Anja Schmidt

Manual isolation of parthenogenetic egg cells combined with comparative transcriptomics to identify parthenogenesis genes in Tripsacum / Xixi Zheng, María Flores-Tornero, and Thomas Dresselhaus

Gene discovery in reproduction : RNA-seq analysis of isolated pistils from apomictic and sexual Eragrostis curvula / Juan Pablo Selva, José Carballo, Diego Zappacosta, and Viviana Echenique

Genetic mapping in apomicts / Ross Bicknell, Andrew Catanach, and Chris Winefield

Chromosome preparation and fluorescent in situ hybridization / Terezie Mandáková

Targeted mutagenesis in natural apomicts / Tatyana Radoeva, Diana Rigola, Rik H. M. Op den Camp, Peter J. van Dijk, and Charles J. Underwood

Map-based cloning of apomixis genes in dandelion (Taraxacum officinale s.l.) / Peter J. van Dijk, Tatyana Radoeva, Rik H. M. Op den Camp, and Diana Rigola

Pilosella : A dicotyledonous model for studying aposporous, autonomous apomixis / Ross Bicknell and Anna M. G. Koltunow

The genus Boechera as a model for apomixis research / Terezie Mandáková

How apomictic and sexual Cenchrus species can help elucidate the mechanisms leading to apospory / Joann A. Conner, Yuji Ke, and Peggy Ozias-Akins

Synthetic apomeiosis in tomato via the mutagenesis of SlSPO11-1, SlREC8, and SlTAM to engineer mitosis instead of meiosis / Yazhong Wang, Iris E. Zahn, Bas Heidemann, Roven Rommel Fuentes, and Charles J. Underwood

Engineering synthetic apomixis in hybrid rice / Donaldo Meynard, Aurore Vernet, Imtiyaz Khanday, Laurine Gilles, Raphaël Mercier, Venkatesan Sundaresan, and Emmanuel Guiderdoni.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 19, 2025).

Other Format:

Print version: Apomixis

ISBN:

9781071650011

1071650017

OCLC:

1552175246

Access Restriction:

Restricted for use by site license