Phytochromes : methods and protocols / edited by Andreas Winkler.

Format:

Book

Contributor:

Winkler, Andreas (Of Technische Universität Graz), editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2970.

Methods in molecular biology, 1940-6029 ; 2970

Language:

English

Subjects (All):

Phytochrome--Laboratory manuals.

Phytochrome.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (xii, 356 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume summarizes the state-of-the-art methodology for the functional characterization of phytochromes in vitro and in vivo. Chapters and divided into four parts covering, phytochrome discovery, spectroscopic techniques, x-ray crystallography, x-ray crystallography, and analyzing phytochrome function in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Phytochromes: Methods and Protocols aims to be a versatile laboratory bench and computational resource that is an essential reference.

Contents:

Discovery and engineering of unique cyanobacteriochrome GAF domains / Takahisa Suzuki, Ayano Yoneda, Tomo Yokota, Hiroki Hoshino, Keita Miyake, Keiji Fushimi, and Rei Narikawa

Analysis of sequence diversity in subfamilies of phytochrome‑linked effectors / Cornelia Böhm and Massimo Totaro

Strategies for coexpression of bilin‑producing enzymes in Escherichia coli / Oliver Maximilian Eder, Marta Jančić, and Andreas Winkler

Preparation of phycocyanobilin (PCB) with and without 13C/15N isotope labeling for in vitro phytochrome autoassembly / Jon Hughes and Christina Lang

Production of plant phytochromes by combining insect cells and bacterial expression systems / Cheng Chi, Zhengdong Wang, Ruihan Wang, and Jizong Wang

Overcoming the challenges of producing plant phytochromes in Escherichia coli / Sintayehu Manaye Shenkutie and Kaoling Guan

Characterizing phytochrome photochemistry using UV–visible spectroscopy in host cell extracts and purified protein / Jimena Rinaldi, Lisandro H. Otero, and Hernán R. Bonomi

Computational protocols to study phytochromes / Probal Nag, Chongyao Wei, Megan J. Mackintosh, and Igor Schapiro

Using computational techniques for the characterization of structural changes during the phytochrome photocycle / Giacomo Salvadori

FTIR spectroscopy of red‑ and far‑red‑light sensing phytochromes in vitro / Moona Kurttila and Janne A. Ihalainen

Spectroscopic analyses of photoconversion in phytochromes / Chengwei Yi, Stefanie S. M. Meier, Maria Kehr, and Andreas Möglich

Small‑angle X‑ray scattering studies on phytochromes / Mao Oide and Masayoshi Nakasako

A simple modification of a commercial plunge‑freezer to allow time‑resolved vitrification for cryo‑EM analyses of light‑activated proteins / Andrea Flores‑Ibarra, Monika Gunkel, and Elmar Behrmann

Structures of myxobacterial phytochrome revealed by cryo‑EM / Tek Narsingh Malla, Emina A. Stojković, and Marius Schmidt

Hydrogen–deuterium exchange coupled to mass spectrometry‑based analysis of phytochrome photoreceptors / Maximilian Fuchs and Andreas Winkler

Encapsulation of phytochrome proteins in trehalose matrices : Practical considerations for use in solid‑state NMR spectroscopy / Valentin Rohr, Wolfgang Gärtner, Jörg Matysik, and Chen Song

Analyzing in vitro autokinase activity of microbial phytochromes under well‑defined light conditions / Christina Huber, Merle Strack, Rolf Diller, and Nicole Frankenberg‑Dinkel

Detection of histidine kinase activities of bacteriophytochromes with Phos‑tag™ acrylamide gel electrophoresis / Iida Tuure and Heikki Takala

Assessing the role of PPKs in phosphorylating phytochrome A using the protoplast transient expression system / Ziyi Feng and Jigang Li

A fast and reliable nuclei extraction method for phytochrome functional analysis / Ilse Villmann, Akira Nagatani, and Mathias Zeidler

Opto‑APCs : Displaying phytochrome B on the surface of living cells for optogenetic studies / Anna K. Ehret, Marissa Russ, O. Sascha Yousefi, and Wolfgang W. Schamel

Coupling recombinant PhyB to cell culture plates and beads / Anna K. Ehret, Lisa Entringer, Anne Smedegaard Frederiksen, Vincent Idstein, Sara Hartmann, and Wolfgang W. Schamel.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 16, 2025).

Other Format:

Print version: Phytochromes

ISBN:

9781071647912

1071647911

OCLC:

1545533545

Publisher Number:

CIPO000299342

Access Restriction:

Restricted for use by site license