Enteric glial cells : methods and protocols / edited by Giuseppe Esposito.

Format:

Book

Contributor:

Esposito, G. (Giuseppe), editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2971.

Methods in molecular biology, 1940-6029 ; 2971

Language:

English

Subjects (All):

Gastrointestinal system--Innervation.

Gastrointestinal system.

Molecular biology--Research--Methodology.

Molecular biology.

Neuroglia.

Gastrointestinal Tract--innervation.

Medical Subjects:

Gastrointestinal Tract--innervation.

Neuroglia.

Physical Description:

1 online resource (xi, 170 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This detailed volume highlights the heterogeneity and plasticity of enteric glial cells and explores their contributions to both GI homeostasis and disease pathophysiology. Through an interdisciplinary approach, the book brings together expertise from neurobiology, immunology, physiology, pharmacology, and pathology to provide a comprehensive perspective on the enteric nervous system and its glial components. It explores electrophysiological techniques, the relationship between enteric glia and the gut microbiota, advances in immunohistochemistry, the influence of the gut-brain axis on neurodegenerative diseases and mood disorders, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Enteric Glial Cells: Methods and Protocols serves as an ideal guide for understanding enteric glia but also for stimulating new research avenues that could lead to breakthroughs in treating a range of gastrointestinal and neurological disorders"-- Springer Nature Link.

Contents:

Introducing enteric glial cells / Giuseppe Esposito

Immunohistochemical localization and anatomical distribution of enteric glia cells in enteric nervous system / Sofia Faggin, Valentina Caputi, Angela Tietto, Maria Cecilia Giron

Immunohistochemical staining protocol of enteric glial cells in myenteric plexi / Aurora Zilli, Silvia Basili Franzin, Luisa Seguella

Rodent enteric glia culture / Jacques Gonzales, Tony Durand, Brian D. Gulbransen

Isolation of distinct populations of enteric glia from the muscularis externa and mucosa of the mouse intestine / Anoohya N. Muppirala, Meenakshi Rao

Isolation and dissection procedures for EGC isolation by human mucosal biopsies / Giovanni Sarnelli, Irene Palenca, Maria Sole Facioni, Pierluigi De Pierro, Marcella Pesce

Differentiating enteric glial cells within intestinal organoids derived from human pluripotent stem cells / Simon Vales, Lola Bonneau, Maxime M. Mahe

Transgenic mouse models to study enteric glia / Wilmarie Morales-Soto

Enteric glial cell culture to mimic in vivo obesity and neurodegenerative disorders / Vanessa D’Antongiovanni, Clelia Di Salvo, Laura Benvenuti, Matteo Fornai

Fluorescence-activated cell sorting of primary enteric glia / Austin Irwin, Anthony Pellicano, Vladimir Grubišic

Methods to isolate enteric glia from hPSC-derived enteric ganglioids / Evgeniia Pimenova, Faranak Fattahi, Homa Majd

Calcium imaging studies in enteric glia / Beatriz Thomasi, Julia Jamka, Brian D. Gulbransen

In vitro manipulation of gene expression in enteric glial cells / Martina Vincenzi

In vitro study of microbiota influence on enteric glial cell physiology / Elena Lucarini, Alessandra Toti

Intracerebral transplantation of enteric glia / Sirine Hacene, Nina Colitti, Isabelle Loubinoux, Carla Cirillo.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 14, 2025).

Other Format:

Print version: Enteric glial cells

ISBN:

9781071647950

1071647954

OCLC:

1545097630

Publisher Number:

CIPO000286398

CIPO000286399

Access Restriction:

Restricted for use by site license