Plant stress memory : methods and protocols / edited by Pankaj Kumar, Mohammad Irfan.

Format:

Book

Contributor:

Kumar, Pankaj (Biotechnology scientist), editor.

Irfan, Mohammad, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 2988.

Methods in molecular biology, 1940-6029 ; 2988

Language:

English

Subjects (All):

Plants--Effect of stress on--Laboratory manuals.

Plants.

Physical Description:

1 online resource (xi, 216 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.

Contents:

Thermomemory assay of Arabidopsis plants / Justyna J. Olas and Philip A. Wigge

Assays to study plant response to high temperature in Arabidopsis: Evidence in glutathione biosynthesis mutants / Juline Auverlot, Jean‑Philippe Reichheld, and Avilien Dard

Application of UV‑A radiation to induce tolerance to biotic and abiotic stress in horticultural crops / Diego Iván Escobar‑Hernández, Yolanda González‑García, Adalberto Benavides‑Mendoza, and Antonio Juárez‑Maldonado

Assessment of transgenerational plasticity of salinity tolerance in rice / Murat Aycan and Toshiaki Mitsui

Studying DNA methylation for transgenerational inheritance / Jong‑Joo Cheong

Single‑base resolution DNA methylation analysis through targeted locus‑specific bisulfite sequencing in plants / Kirti Pandey and Gaurav Zinta

Targeted bisulfite sequencing and DNA methylation analysis in rice endosperm and other tissues / Avik Kumar Pal, Vivek Hari‑Sundar Gandhivel, and P. V. Shivaprasad

Immunofluorescence staining and microscopic imaging of plant nuclei for epigenetic modifications / Vivek Hari‑Sundar Gandhivel, Steffi Raju, and P. V. Shivaprasad

iTRAQ‑based proteomic approach to study stress memory responses in plants / Monika Chouhan, Paramdeep Kumar, and Rajiv Kumar

Mass spectrometry‑based methods for plant metabolite profiling / Ashwani Punia, Satyakam, and Rajiv Kumar

Posttranslational modifications in proteins under stress and memory / Twinkle, Dipanshu Ghosh, Sandhya Yadav, and Vivek Dogra

RNA‑seq analysis in plant stress memory: A bioinformatics protocol / Shivanti Negi, Shagun Sharma, Pankaj Kumar, and Mohammad Irfan

Target‑specific single guide RNA (sgRNA) design and in vitro validation of target‑specific sgRNAs for CRISPR/Cas9‑mediated editing of a plant stress memory‑associated gene / Pankaj Kumar, Vipasha Verma, and Mohammad Irfan

Studying heat‑stress memory in red algae / Koji Mikami and Megumu Takahashi

Method to study induced systemic resistance against bacterial pathogens in orchids primed by root colonization of mycorrhizal fungi / Galih Chersy Pujasatria, Chihiro Miura, and Hironori Kaminaka

Priming seeds and plants with microbial inoculants to improve growth and stress memory / Rahul Thakur and Saurabh Yadav

Quantification of jasmonic acid and salicylic acid in plant–insect interactions / Mukesh Kumar Meena

Quantifying arbuscular mycorrhizal fungal colonization via anthocyanin pigmentation in Medicago truncatula roots / Anil Kumar, Fuyu Li, and Qiuju Li

Insect herbivory simulation and insect bioassays to study plant stress memory response / Mukesh Kumar Meena.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed January 29, 2026).

Other Format:

Print version: Plant stress memory

ISBN:

9781071649817

1071649817

OCLC:

1547388193

Access Restriction:

Restricted for use by site license