Pichia pastoris : methods and protocols / edited by Xavier García-Ortega, Anton Glieder, Karin Kovar, Lukas Rieder.

Format:

Book

Contributor:

Garcia-Ortega, Xavier, editor.

Glieder, Anton, editor.

Kovar, Karin, 1967- editor.

Rieder, Lukas, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2697.

Methods in molecular biology, 1940-6029 ; 2697

Language:

English

Subjects (All):

Pichia pastoris--Laboratory manuals.

Pichia pastoris.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (xvii, 682 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pichia pastoris: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

Contents:

A brief history of the Pichia pastoris expression system / James M. Cregg, Thomas G. Chappel, Kurt R. Gehlsen, Shaun A. Kirkpatrick, and Knut Madden

Pichia pastoris genomic resources: Accessibility, recommendations, and pitfalls / Veronika Schusterbauer, Lena Schaffer, and Gerhard G. Thallinger

Isolation of Pichia pastoris genomic DNA for long‑read DNA sequencing and PCR applications / Sven Heimhilcher, David Keßler, and Lukas Rieder

Pichia pastoris RNA‑sequencing and data analysis / Julian Christopher Krappinger, Astrid Radkohl, Veronika Schusterbauer, Anita Emmerstorfer‑Augustin, and Julia Feichtinger

Localization of proteins in the secretory pathway of Pichia pastoris using fluorescence microscopy / Verena Puxbaum, Brigitte Gasser, and Diethard Mattanovich

Auxin‑induced degradation of target proteins in Pichia pastoris / Leonie Lehmayer, Lukas Bernauer, and Anita Emmerstorfer‑Augustin

Establishing reference genes for Pichia pastoris quantitative real‑time polymerase chain reaction analyses / Kai Büchner, Lana Vidal, Roland Kerpes, and Thomas Becker

ID‑MS‑based quantitative analysis of metabolites in Pichia pastoris: A step‑by‑step protocol / Marc Carnicer, S. Aljoscha Wahl, Reza Maleki Seifar, Joan Albiol, Walter van Gulik, and Pau Ferrer

High‑throughput stationary 13C‑based metabolic flux analysis of Pichia pastoris strains / Albert Fina, Pierre Millard, Cecilia Berges, Pau Ferrer, Joan Albiol, and Stéphanie Heux

Determination of biomass elemental composition of Pichia pastoris / Arnau Gasset, Francisco Valero, and José Luis Montesinos‑Seguí

Pichia pastoris expression plasmid assembly using type IIS restriction enzymes / Alexandra Charlotte Ilse Grebe, Lia Jade Schallengruber, Carla Magdalena Aguilar Gomez, and Lukas Rieder

Autonomously replicating sequence‑bearing plasmids utilized in Pichia pastoris / Carsten Pichler, Florian Weiss, and Anton Glieder

Overlap extension construction and direct cloning of loop‑out prone DNA expression cassettes in Pichia pastoris: 75 k‑γ‑secalin from Secale cereale (rye) as a case study / Kai Büchner, Carsten Pichler, Roland Kerpes, and Thomas Becker

In vivo plasmid assembly in NHEJ‑deficient Pichia pastoris strains / Leon Wolbank, Florian Weiss, and Anton Glieder

Protein targeting signals and strategies for Pichia pastoris / Sarah Gangl and Katharina Ebner

Cloning of MFα secretion signal variants for protein secretion by Pichia pastoris / Magdalena Merkaš, Odyssefs‑Ioannis Pantelakis, and Anita Emmerstorfer‑Augustin

Lytic polysaccharide monooxygenases as reporters for signal peptide evaluation in Pichia pastoris / Andrea K. Hönikl and Lukas Rieder

Exploiting self‑processing 2A peptides for multigene expression in Pichia pastoris / Katharina Ebner and Martina Geier

Retrofitting of AOX1 promoter‑based Pichia pastoris expression strains for methanol‑independent protein production / Tanja Michelle Schleichert, Lisa Wasserer, and Anton Glieder

Selective cultivation of methylotrophic Pichia pastoris laboratory and natural strains / Sarah Gangl, Marina Schmid, Maik Hilgarth, and Katharina Ebner

Transformation of Pichia pastoris by electroporation / Kirill Smirnov and Anton Glieder

High‑throughput transformation of chemical competent Pichia pastoris cells using lithium acetate/chloride and carrier DNA / Kirill Smirnov, Amina Hasanagic, Lukas Rieder, and Anton Glieder

High‑efficiency transformation of integrative libraries under zeocin selection in Pichia pastoris / Morgane Boone, Hannah Eeckhaut, and Nico Callewaert

High‑throughput generation of Pichia pastoris knock‑out strains by using CRISPR/Cas9 / Kirill Smirnov, Lukas Rieder, and Anton Glieder

Engineering Pichia pastoris strains using CRISPR/Cas9 technologies: The basic protocol / Hana Raschmanová, Astrid Weninger, and Karin Kovar

Adaptive laboratory evolution of Pichia pastoris by serial batch cultivation / Charles Moritz, Thomas Gassler, Martin Altvater, and Diethard Mattanovich

Classical genetics of Pichia pastoris / Ilya Tolstorukov, Thomas G. Chappell, and James M. Cregg

Pichia pastoris random mutagenesis and screening / Christine Winkler, Joan Lin‑Cereghino, and Geoff P. Lin‑Cereghino

Cryopreservation of Pichia pastoris strains for long‑term storage / Pierre D. M. Reinprecht and Katharina Ebner

Adapting Komagataella phaffii cultivation media to REACH regulations / Julian Kopp, Alexander Pekarsky, and Oliver Spadiut

Fed‑batch operational strategies for efficient and robust Pichia pastoris cultivations achieving high cell densities / Albert Sales‑Vallverdú, Miguel Angel Nieto‑Taype, Arnau Gasset, José Luis Montesinos‑Seguí, Francisco Valero, and Xavier Garcia‑Ortega

A guide to fed‑batch cultivation of Pichia pastoris on laboratory scale / Marcel Straumann, Hana Raschmanová, Valentina Haag, Iwo Zamora, and Karin Kovar

Pichia pastoris continuous cultivations for strain characterization and continuous biomanufacturing / Núria Bernat‑Camps, Arnau Gasset, José Luis Montesinos‑Seguí, Francisco Valero, and Xavier Garcia‑Ortega

Best practices for protein expression in Pichia pastoris combining design of experiments with an automated platform for parallelized cultivation in small‑scale stirred tank bioreactors / Holger Morschett, Joan Cortada‑Garcia, Hauke Holm, Núria Adelantado, and Karlheinz Flicker

Kinetic modeling of Pichia pastoris bioreactor systems under different operating conditions: Batch, fed‑batch, and chemostat / Julio Berrios, Carlos Martínez, and Patrick Fickers

Online monitoring and control of methanol concentration in Pichia pastoris fermentations / Francisco Valero, José Luis Montesinos‑Seguí, and Arnau Gasset

Off‑gas balancing for bioprocess monitoring Pichia pastoris bioprocesses / Lisa Michel, Gesine Cornelissen, and Christian Kaiser

A single‑use technology platform: How to set up bioprocesses with Pichia pastoris / Melanie Ottinger and Jason Brown

A Pichia pastoris cell‑free protein synthesis platform / Rochelle Aw, Farzana Alam, Alex J. Spice, and Karen M. Polizzi

Clarification of Pichia pastoris broth by TFF with ceramic membranes / Fuensanta Verdú‑Navarro, Juan A. Moreno‑Cid, Ana G. Del Hierro, Xavier Garcia‑Ortega, and Ruth Ordoñez

Pichia pastoris cell disruption by high‑pressure homogenization (HPH) / Antoni Casablancas, Xavier Garcia‑Ortega, and Francisco Valero

Concentration and purification of proteins produced by Pichia pastoris by TFF with ceramic membranes / Fuensanta Verdú‑Navarro, Juan A. Moreno‑Cid, Ana G. Del Hierro, Xavier Garcia‑Ortega, and Ruth Ordoñez

Protein purification protocols for recombinant enzymes produced in Pichia pastoris / Merve Keser, Mikel Dolz, Javier Viña‑Gonzalez, David Gonzalez‑Perez, and Miguel Alcalde

Characterization of low molecular weight variants (fragmentation) in Pichia pastoris by CE‑SDS / Rocío Cuevas Casado and Albert Font Inglès.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 16, 2025).

Other Format:

$i Print version: Pichia pastoris

ISBN:

9781071647790

1071647792

OCLC:

1545533560

Publisher Number:

CIPO000299340

Access Restriction:

Restricted for use by site license