SMC proteins : methods and protocols / edited by Yasuto Murayama, Yasutaka Kakui.

Format:

Book

Contributor:

Murayama, Yasuto, editor.

Kakui, Yasutaka, editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in Molecular Biology, 1940-6029 ; 2991

Language:

English

Subjects (All):

Single cell proteins--Laboratory manuals.

Single cell proteins.

Genre:

Laboratory manuals

Physical Description:

1 online resource (x, 276 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

The aim of this volume is to provide a wide range of experimental methods for analyzing molecular functions of SMC complexes, such as microscopic imaging, protein localization, chromosome conformation analyses by deep DNA sequencing and biochemical reconstitution with purified proteins or cell lysate. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, SMC Proteins: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Contents:

Single-molecule analyses of human cohesin complex / Yoshimi Kinoshita and Tomoko Nishiyama

Interaction between an individual cohesin complex and DNA revealed by optical tweezers and single-molecule microscopy / Martina Richeldi and Maxim Molodtsov

Visualizing binding of single SMC complexes to DNA substrates using combinational optical tweezers and fluorescence microscopy / Anno I. Koetje, Gemma L. M. Fisher, Menelaos Voulgaris, Sanjana Saravanan, Richard J. Bartlett, Stephen J. Cross, and Luis Aragon

Atomic force microscopy analysis of bridging-induced phase separation mediated by SMC proteins / Sara Illodo, Hyeong Ku Kim, and Je-Kyung Ryu

Monitoring local chromatin dynamics regulated by SMC complexes in fission yeast / Yasutaka Kakui

DNA bioconjugation with polymer beads using SNAP-tag® technology / Hon Wing Liu and Stephan Gruber

Gel electrophoresis analysis of SMC-mediated effects on DNA topology / Joaquim Roca

The use of the homobifunctional crosslinker BMOE to investigate cohesin function by measuring protein–protein and protein–DNA interactions / James Collier and Madhusudhan Srinivasan

Bulk FRET analysis of the conformation of the cohesin ring / Torahiko L. Higashi

Assessing SMC complex function in replication fork progression with DNA fiber assays / Neus P. Lorite, Snehlata, Neus Colomina, and Jordi Torres-Rosell

Biochemical reconstitution of replication-coupled cohesin acetylation / Masashi Minamino, Céline Bouchoux, and Frank Uhlmann

A biochemical assay to monitor the encounter between cohesin and DNA replication / Yasuto Murayama

An in vitro assay to study RSC remodeling activity stimulation by the cohesin loader / Sofía Muñoz

Purification and in vitro analysis of Escherichia coli RecN / Shunsuke Noda and Takashi Hishida

Production and purification of the human SMC5/6 core complex from S. cerevisiae / Hemanta Adhikary and Damien D’Amours

ChIA-PET analysis for investigating SMC-mediated 3D genomic contacts / Kyoung-Dong Kim and Ken-ichi Noma

High-resolution chromosome conformation capture method to study archaeal SMC complexes / Kodai Yamaura and Naomichi Takemata.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 12, 2025).

Other Format:

Print version: SMC proteins

ISBN:

9781071650059

107165005X

OCLC:

1550521293

Access Restriction:

Restricted for use by site license