Single-Cell Omics in Microbiome Research : Exploring Microbial Diversity Across Ecosystems / edited by Christian Rinke, Mária Džunková.

Format:

Book

Author/Creator:

Rinke, Christian.

Series:

The Microbiomes of Humans, Animals, Plants, and the Environment, 2662-6128 ; 7

Language:

English

Subjects (All):

Microbial ecology.

Microbiology--Technique.

Microbiology.

Microbial genetics.

Environmental Microbiology.

Microbiology Techniques.

Microbial Genetics.

Local Subjects:

Environmental Microbiology.

Microbiology Techniques.

Microbial Genetics.

Physical Description:

1 online resource (186 pages)

Edition:

1st ed. 2025.

Place of Publication:

Cham : Springer Nature Switzerland : Imprint: Springer, 2025.

Summary:

This volume introduces the revolutionary field of microbial single-cell omics, a suite of cutting-edge techniques that uncover the genetic, functional, and metabolic signatures of individual microbial cells. From the discovery of new microbial lineages to decoding virus-host interactions, this book highlights the transformative impact of single-cell genomics, transcriptomics, proteomics, and metabolomics. With expert protocols and innovative workflows, the book provides practical guidance on fluorescence-activated cell sorting (FACS), microfluidic encapsulation, and advanced genome amplification techniques. It also explores targeted methods, like FISH-FACS for recovering rare microbes, activity-based cell separation, and multi-omics approaches that integrate genomic, transcriptomic, and proteomic data. The authors discuss future developments and the immense benefits of analysing and understanding microbes one cell at a time, offering a glimpse into how this knowledge can transform microbial ecology, biomedicine, and biotechnology. The book is aimed at researchers and students of environmental microbiology who wish to expand their knowledge of this ground-breaking approach and the underlying technology.

Contents:

Chapter 1: A Hitchhikers Guide to Microbial Single-Cell Omics

Chapter 2: Overview of laboratory workflows for microbial single-cell genomics

Chapter 3: Characterization of microbial diversity and mobile genetics elements through single-cell genomics

Chapter 4: FISH-FACS enabled targeted recovery of genomes from uncultivated environmental microbial populations

Chapter 5: Advancements in the application of activity-based studies for separation of microbial populations via fluorescence-activated cell sorting

Chapter 6: Technical advancements in microbial single-cell omics analysis

Chapter 7: Challenges and Future Perspectives for Single-Cell Multi-Omics in Prokaryotes

Chapter 8: Single-Cell Sorting to Assess Phage Sensitivity in Heterogeneous Bacterial Populations.

Notes:

Description based on publisher supplied metadata and other sources.

Other Format:

Print version: Rinke, Christian Single-Cell Omics in Microbiome Research

ISBN:

9783032075277

OCLC:

1572217398