Enzymes of polyamine metabolism / edited by Robert A. Casero Jr., Tracy Murray Stewart.

Format:

Book

Contributor:

Casero, Robert Anthony, editor.

Stewart, Tracy Murray, editor.

Series:

Methods in enzymology ; v. 715.

Methods in enzymology, 0076-6879 ; 715

Language:

English

Subjects (All):

Polyamines--Metabolism.

Polyamines.

Enzymes.

enzyme.

Physical Description:

xxx, 458 pages : illustrations 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, 2025.

Summary:

Enzymes of Polyamine Metabolism, Volume 715 highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Convergence of MYC and the hypusine circuits in the control of metabolism, HDAC10 structure and enzymology, TR-FRET Assay for Profiling HDAC10 Inhibitors and PROTACs, Polyamine Transport Inhibitors: Methods and caveats associated with measuring polyamine uptake in mammalian cells, Evaluation of platinum drug toxicity resulting from polyamine catabolism, Measurements of acrolein adducts resulting from polyamine catabolism, Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori: implications for gastric inflammation and carcinogenesis, and much more. Other sections delve into Molecular and biochemical analysis of eIF5A, Analysis of in vivo SMOX activity, Structure and role of ATP13A2 in polyamine transport, Analysis of Translational Regulation Using Polysome Profiling and Puromycin Incorporation, Targeting polyamine metabolism in an ex vivo prostatectomy model, Development and characterization of a Drosophila model of SRS, Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples, Gene replacement therapy to restore polyamine metabolism, Investigation of polyamine metabolism in bone homeostasis, Collection, preparation, and biobanking of clinical specimens for polyamine analysis, and many other topics.

Contents:

Genetic analyses of Myc and hypusine circuits in tumorigenesis

Expression, purification, and crystallization of "humanized" Danio rerio histone deacetylase 10 "HDAC10", the eukaryotic polyamine deacetylase

TR-FRET assay for profiling HDAC10 inhibitors and PROTACs

Polyamine transport inhibitors : methods and caveats associated with measuring polyamine uptake in mammalian cells

Evaluation of platinum drug toxicity resulting from polyamine catabolism

Measurements of acrolein adducts resulting from polyamine catabolism

Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori : implications for gastric inflammation and carcinogenesis

Yeast reconstituted translation assays for analysis of eIF5A function

Quantification of spermine oxidase (SMOX) activity in tissues by HPLC

Direct measurement of ATP13A2 polyamine-dependent ATPase activity following rapid purification of lysosomes

Analysis of translational regulation using polysome profiling and puromycin incorporation

Targeting polyamine metabolism in an ex vivo prostatectomy model

Development and characterization of a Drosophila model of Snyder-Robinson syndrome

Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples

Gene replacement therapy to restore polyamine metabolism in a Snyder-Robinson syndrome mouse model

Methods to study polyamine metabolism during osteogenesis

Collection, preparation, and biobanking of clinical specimens for analysis in polyaminopathies

An enhanced method for the determination of polyamine content in biological samples by dansyl chloride derivatization and HPLC

Cell-based determination of HDAC10-mediated polyamine deacetylase activity

Drosophila melanogaster imaginal disc assays to study the polyamine transport system

A fluorescence-based assay for measuring aminopropyltransferase activity

Isolation of Hafnia paralvei FB315, a polyamine-high-producing bacterium, from aged cheese

Methylated polyamines derivatives and antizyme-related effects

Novel LC-MS/MS assay to quantify D,L-alpha-difluoromethylornithine (DFMO) in mouse plasma

Polyamine quantitation by LC-MS using isobutyl chloroformate derivatives.

ISBN:

9780443317842

0443317844

OCLC:

1536374857