Lipases : Foundations, Applications, and Perspectives.

Format:

Book

Author/Creator:

Cipolatti, Eliane Pereira.

Contributor:

Manoel, Evelin Andrade.

Oliveira, Débora De.

Series:

Foundations and Frontiers in Enzymology Series

Language:

English

Physical Description:

1 online resource (510 pages)

Edition:

1st ed.

Place of Publication:

Chantilly : Elsevier Science & Technology, 2025.

Summary:

Lipases: Foundations, Applications, and Perspectives, a new volume in the Foundations and Frontiers of Enzymology series, offers a practical, in-depth discussion of lipases and instruction in their use across research and industry.

Contents:

Front Cover

Lipases: Foundations, Applications, and Perspectives

Copyright Page

Dedication

Contents

List of contributors

About the editors

1 Introduction

1 Lipases: from the past to the future

1.1 Introduction

1.2 Lipases in the past: a brief history

1.3 Classification of lipases

1.4 Activity of lipases

1.5 Structure of lipases

1.5.1 The binding sites

1.5.2 The catalytic machinery

1.5.3 The lid

1.5.4 The lid hinge

1.6 Efficiency of lipases as biocatalysts

1.6.1 Efficiency related to lipase structure

1.6.2 Efficiency in mild conditions

1.6.3 Efficiency in extreme conditions

1.6.4 Techniques to improve lipase efficiency

1.7 The future impact of lipases on the industrial sector

1.8 The lipase industry: market overview, trends, and technological advancements

1.8.1 Global enzyme market and lipase industry overview

1.8.2 Lipase market dynamics, global distribution, key players, and patent trends

1.9 Final considerations and future prospects

Acknowledgments

References

2 Working with lipases: fundamental aspects

2.1 Introduction

2.2 The essential elements of catalysis

2.2.1 Enzyme activity measurement techniques

2.2.2 The use of natural and artificial substrates

2.3 Specificity of lipases

2.4 Influence of water addition on organic catalysis with lipases

2.5 Designing lipases for improved catalytic activity

2.6 Conclusion

3 Working with lipases: analytical techniques

3.1 Introduction

3.1.1 Lipases definition, structure, and activity

3.1.1.1 Lipases activity determination

3.2 Methods and procedures

3.2.1 Qualitative methods

3.2.1.1 Solid media methods

3.2.1.2 Zymograms

3.2.1.3 Other qualitative methods

3.3 Quantitative methods.

3.3.1 Titrimetric method: the conventional but still powerful approach

3.3.2 Spectrometric methods: a wide variety of convenient techniques

3.3.2.1 Colorimetry

3.3.2.2 Visible spectrophotometry

3.3.2.3 Infrared spectroscopy

3.3.3 Fluorometric and chemiluminescent methods

3.3.3.1 Fluorometric methods

3.3.3.2 Chemiluminescent methods

3.3.4 Turbidimetry

3.3.5 Conductometric method

3.3.6 Electrochemical method

3.3.7 Chromatographic techniques

3.4 Biosensors

3.5 Conclusion

4 The complexity of interfacial activation and the presence of lids on lipases

4.1 Introduction

4.2 The interfacial activation phenomena and the effects of surfactants

4.3 Lipases structural features and lid domain diversity

4.4 Molecular dynamics for function elucidation

4.4.1 MD for the investigation of Thermomyces lanuginosus lipase

4.4.2 MD for the investigation of the Pseudomonas aeruginosa lipase

4.4.3 MD for the Investigation of the porcine pancreatic lipase

4.5 No lid, and yet interfacial activation?

4.6 Conclusion

AI disclosure

2 Sources and production of lipases

5 Production of microbial lipases

5.1 Introduction

5.2 Microbial lipase sources

5.2.1 Filamentous fungi lipases

5.2.2 Yeast lipases

5.2.3 Bacterial lipases

5.3 Factors influencing microbial lipase production

5.3.1 Substrates

5.3.2 Temperature

5.3.3 pH

5.3.4 Agitation and aeration

5.4 Agro-industrial waste

5.5 Limitations

5.6 Conclusion

6 Biomass-derived substrates for lipase production: Strategies and potential

6.1 Introduction

6.2 Biomass diversity and its potential as substrates for lipase production

6.3 Factors determining the production of lipases with biomass

6.4 Downstream steps.

6.4.1 Extraction process of extracellular lipases

6.4.1.1 Soaking time

6.4.1.2 Temperature

6.4.1.3 Dimethyl sulfoxide

6.4.1.4 Glycerol

6.4.1.5 Buffer and pH

6.4.1.6 Detergents

6.4.1.7 Sodium chloride (NaCl)

6.4.1.8 Alternative approaches

6.4.2 Purification

6.5 Circular economy, bioeconomy, and circular bioeconomy applied to lipase production

6.5.1 Defining circular economy, bioeconomy, and circular bioeconomy

6.5.2 The importance of circular economy, bioeconomy, and circular bioeconomy

6.5.3 Relationship between circular bioeconomy and biomass utilization for lipase production

6.6 Conclusion

7 Production of optimized biocatalysts with immobilized lipases

7.1 Introduction

7.2 Lipases

7.3 Fundamentals and advantages of enzyme immobilization

7.4 Overview of immobilization methods: binding, encapsulation, and enzymatic cross-linking

7.4.1 Binding of enzymes to supports

7.4.1.1 Immobilization by physical adsorption

7.4.1.2 Immobilization by ionic interactions

7.4.1.3 Immobilization by covalent bonds

7.4.1.4 Immobilization by encapsulation

7.4.1.5 Immobilization by cross-linking

7.5 Impact of distinct aspects on the performance of immobilized lipase

7.5.1 Structural and environmental effects on lipase performance

7.5.2 Partition effects and their influence on enzyme activity

7.5.3 Diffusional limitations in immobilized systems

7.5.4 Effect of ionic strength on immobilization efficiency

7.5.5 Effect of pH and temperature on enzyme stability

7.6 Impact of distinct aspects on the stability of immobilized lipase

7.6.1 Factors influencing enzyme stability postimmobilization

7.6.2 Strategies to enhance stability of immobilized lipases

7.7 Impact of support features on immobilized lipase performance.

7.7.1 Role of support characteristics in immobilization efficiency

7.8 Polymeric supports for enzyme immobilization

7.8.1 Types and properties of polymeric supports

7.8.2 Applications of polymeric supports in biocatalysis

7.9 Final considerations and perspectives

3 Biotechnological applications

8 Top industrially applied enzymes

8.1 Introduction

8.2 Main origins of industrial lipases

8.3 Top application of lipases in industry

8.3.1 Using lipases to synthesize biodegradable polymers

8.3.2 Use of lipases to degrade plastics and microplastics

8.3.3 Method for obtaining monomers from acidic waste generated during cyclohexane oxidation

8.3.4 Use of lipases in biomass valorization processes

8.3.5 Use of lipases to produce diols during the alcohol fermentation processes

8.3.6 Use of lipases in the in the generation of products with greater added value from oily waste

8.3.7 Use of the lipases on preparation of sensors for smart packaging

8.3.8 Lipases for prevention and removal of biofilm

8.3.9 Paint removal

8.3.10 Lipases on the production of zootechnical additives in animal feed

8.4 Perspectives and conclusions

9 The future of lipases in biofuel and biolubricant synthesis

9.1 Introduction

9.2 Biodiesel

9.2.1 Biocatalysts-free, immobilized, whole-cell, and dry fermented solids

9.2.2 Feedstocks

9.2.3 Scale-up and economic aspects

9.3 New trends and lipase-based initiatives for biofuels production

9.4 Biolubricants

9.5 Conclusion and perspectives

Conflict of interest

10 Lipases in the modification of oils and fats in the food industry

10.1 Introduction

10.2 Lipases as biocatalysts for the oils and fats modification industries

10.3 Processes for modifying oils and fats.

10.3.1 Products obtained in aqueous media

10.3.2 Products obtained in organic media

10.3.3 Esterification products

10.3.4 Interesterification products

10.4 Applications and trends

10.4.1 Cocoa butter substitutes

10.4.2 Human milk fat substitutes

10.4.3 Low-calorie structured lipids

10.4.4 Edible film applications

10.5 Conclusions

11 Lipases in the pharmaceutical and cosmetic industries

11.1 Introduction

11.2 Lipase as a biocatalyst in the pharmacological industry

11.2.1 Antidepressants

11.2.2 Nonsteroidal antiinflammatory drugs

11.2.3 Antibiotics

11.3 Inhibition of gastric and pancreatic lipases

11.4 Lipases in the cosmetics industry

11.5 Lipases in biosensors and diagnostics

11.6 Conclusion

12 Treatment of industrial waste using lipases

12.1 Introduction

12.2 Lipids

12.2.1 Lipids in wastewater: sources and behavior

12.2.2 How to remove lipids?

12.2.2.1 Mechanical and chemical methods

12.2.2.2 Using biological methods

12.3 Biocatalysis of the whole cell

12.3.1 The use of crude lipase preparation

12.3.2 Immobilized lipases

12.4 The combination of enzyme and biosurfactant for wastewater treatment

12.5 Wastewater treatment for value-added products using enzymes and microorganisms

12.6 Lipase enzyme applications

12.6.1 Lipase application in wastewater treatment

12.6.2 The advantages of lipase for wastewater treatment

12.7 The lipase's limitations and future perspective in the wastewater treatment

Index

Back Cover.

Notes:

Description based on publisher supplied metadata and other sources.

ISBN:

0-443-13309-3

9780443133091

OCLC:

1545645645