Inositol pyrophosphates methods and protocols edited by Adolfo Saiardi, Dorothea Fiedler, Henning J. Jessen

Format:

Book

Contributor:

Saiardi, Adolfo, editor.

Fiedler, Dorothea, editor.

Jessen, Henning J., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology 1940-6029

Language:

English

Subjects (All):

Inositol phosphates--Laboratory manuals.

Inositol phosphates.

Pyrophosphates--Laboratory manuals.

Pyrophosphates.

Physical Description:

1 online resource

Place of Publication:

Springer US, 2025.

New York, NY Humana Press 2025

Summary:

This detailed volume explores methods that reflect the explosion of interest in the important cellular messengers known as inositol pyrophosphates. After a brief historical perspective of the analytical approaches that have contributed to the development of this research field, the book collects methods that cover isolation, detection, and measurement of inositol pyrophosphates, followed by approaches to ascertain their mechanism of action, as well as a description of the use of experimental models that have been instrumental to many fundamental discoveries. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Inositol Pyrophosphates: Methods and Protocols serves as an ideal guide for researchers attempting to further our knowledge of this vibrant area of study

Contents:

Enabling Technologies for the Dissection of Inositol Pyrophosphate Physiology

Cell Extraction and Enrichment of Inositol Polyphosphate and Inositol Pyrophosphates Using Titanium Dioxide

Analysis of Inositol Phosphates via Polyacrylamide Gel Electrophoresis (PAGE)

Enzymatic Synthesis of Inositol Pyrophosphates

Determination of Steady-State Inositol Phosphate Profile of the Liverwort, Marchantia polymorpha Using SAX-HPLC

Analysis of Inositol Phosphates, Diphosphoinositol Phosphates, and Their Analogues by HPLC on an Acid-Compatible Stationary Phase by Complexation with Ferric Ion

Using HILIC-MS/MS Method to Study Inositol Pyrophosphate

Capillary Electrophoresis Mass Spectrometry for Inositol (Pyro)Phosphate Profiling

Absolute Configurations of Inositol Poly- and Pyrophosphates Assigned by Nuclear Magnetic Resonance Spectroscopy

(PP)-InsP Affinity Probes for Target Characterization by Immunoblotting and Mass Spectrometry

Enrichment Workflow for Pyrophosphoproteome Analysis

The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions

Inositol Phosphate Kinase Architecture: Practical Approaches and Lessons Learned

Visualizing and Identifying Inositol Pyrophosphate Isomers In Crystallo

An In Vivo Yeast-Based Activity Assay for the PPIP5K Family

Hydroponic Cultivation Systems to Study Nutrient-Dependent Changes in Inositol Polyphosphate Metabolism in Plants

A Simple RUBY Reporter System in Nicotiana benthamiana for Studying Inositol Pyrophosphate Dynamics

Knockout Mice as an Experimental Model to Study the Biology of Inositol Pyrophosphates

IP6 Kinase Inhibitor Screening Assays

Notes:

Includes index

Online resource; title from PDF title page (SpringerLink, viewed September 11, 2025)

ISBN:

9781071647998

1071647997

OCLC:

1535775980

Access Restriction:

Restricted for use by site license