The nuclear membrane : methods and protocols / edited by Yuki Hara, Kazunori Kume.

Format:

Book

Contributor:

Hara, Yuki, 1982- editor.

Kume, Kazunori, editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology, 1940-6029

Language:

English

Subjects (All):

Nuclear membranes--Laboratory manuals.

Nuclear membranes.

Physical Description:

1 online resource (xi, 346 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This book compiles protocols that encompass a diverse range of classical and contemporary approaches, including biochemical techniques, proteomic analyses, microscopic observations, and biophysical measurements. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Nuclear Membrane: Methods and Protocols aims to be a valuable resource for scientists seeking to explore the nuclear structure and functions in the postgenomic era"-- Springer Nature Link.

Contents:

Lipidomic profiling of isolated nuclei from mice and cultured cells / Nityanand Bolshette, Hanmei Bao, Xianlin Han, and Gad Asher

Profiling plant nuclear envelope composition using subtractive proteomics / Xiao Liu and Yu Tang

Ultrastructural visualization of the nuclear envelope in HeLa cells / Sourabh Sengupta, Zhaojie Zhang, and Daniel L. Levy

Imaging nuclear envelopes using correlative AFM/fluorescence microscopy / Emilie Costes, Anthony Vial, Christine Doucet, and Pierre-Emmanuel Milhiet

Fast super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum dynamics / Swapnil Sahoo and Abdur Rahaman

Immunostaining the yeast nuclear membrane for imaging by super-resolution fluorescence microscopy / Emily M. Sontag

Visualizing phosphatidic acid and diacylglycerol at the nuclear envelope in fission yeast / Sherman Foo and Snezhana Oliferenko

Measuring molecular mass densities at subcellular resolution using optical diffraction tomography / Kyoohyun Kim, Abin Biswas, Jochen Guck, and Simone Reber

Quantification and comparison of protein distribution on the nuclear membrane / Paulina Nastały and Paolo Maiuri

Quantifying nuclear shape fluctuations during early mitosis / Viola Introini, Giancarlo Porcella, Gururaj Rao Kidiyoor, Pietro Cicuta, and Marco Cosentino Lagomarsino

Micropost arrays to model ECM fiber obstacles during cell migration in confinement / Aditya Katiyar, Richard B. Dickinson, and Tanmay P. Lele

Use of nucleoporin-conjugated beads to study the nuclear pore complex assembly on the nuclear membrane / Şükriye Bilir, Yasushi Hiraoka, and Tokuko Haraguchi

T4 DNA-induced reconstruction of artificial nuclei in living mouse oocytes / Nao Yonezawa, Yasushi Hiraoka, Tokuko Haraguchi, and Kazuo Yamagata

Preparation of nucleoplasmic extract and its application in DNA end processing / Daichi Nishiguchi, Kensuke Tatsukawa, and Tatsuro S. Takahashi

Liposome floatation assays to study membrane interactions of nucleoporins / Marianna Tatarek-Nossol and Wolfram Antonin

Giant unilamellar vesicles (GUVs) to study membrane interaction of nucleoporins / Marianna Tatarek-Nossol and Wolfram Antonin

One-pot reconstitution of GPCRs into unilamellar vesicles for fluorescence-based phospholipid scramblase activity assay / Indu Menon and Anant K. Menon

Identifying genomic DNA sequences near the nuclear lamina using proximity biotinylation with ascorbate peroxidase / Yixian Zheng, Katherine A. Bossone, Sara Debic, Ross T. A. Pedersen, and Joseph R. Tran

Pairing lamin B1-DamID and immuno-3D-FISH to resolve and verify peripheral genome organization of adipogenesis / Rafal Czapiewski, Jose I. de las Heras, and Eric C. Schirmer

Measurement of spatial contact map using sequential FISH / Hiroaki Ohishi.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed November 11, 2025).

Other Format:

Print version: Nuclear membrane

ISBN:

9781071647141

1071647148

OCLC:

1535213424

Publisher Number:

CIPO000284723

Access Restriction:

Restricted for use by site license