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Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria / by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino.
- Format:
- Book
- Government document
- Author/Creator:
- Stratis-Cullum, Dimitra N.
- Series:
- ARL-TR (Aberdeen Proving Ground, Md.) ; 5015.
- ARL-TR ; 5015
- Language:
- English
- Subjects (All):
- Campylobacter jejuni.
- Electrophoresis.
- Immunoassay.
- Medical Subjects:
- Campylobacter jejuni.
- Electrophoresis.
- Immunoassay.
- Physical Description:
- 1 online resource (vi, 14 pages) : illustrations (some color).
- Place of Publication:
- Adelphi, MD : Army Research Laboratory, [2009]
- Summary:
- In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.
- Notes:
- Title from title screen (viewed on Nov. 11, 2012).
- "November 2009."
- Includes bibliographical references (pages 9-12).
- OCLC:
- 713205230
- Access Restriction:
- APPROVED FOR PUBLIC RELEASE.
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