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Development of a high-performance gel filtration chromatography polysaccharide sizing method to facilitate glycoside hydrolase functional analysis / Lisa M. Koch, Casey Crooks, Franz J. St. John.
- Format:
- Book
- Government document
- Author/Creator:
- Koch, Lisa M., author.
- Crooks, Casey, author.
- St. John, Franz J., author.
- Series:
- Research note FPL ; 0427.
- Research note FPL ; RN-0427
- Language:
- English
- Subjects (All):
- Glycosidases.
- Hydrolases.
- Gel permeation chromatography.
- Polysaccharides.
- Hemicellulose.
- Xylans.
- Enzymes.
- enzyme.
- Physical Description:
- 1 online resource (7 pages) : color illustrations.
- Place of Publication:
- Madison, WI : Forest Service, U.S. Department of Agriculture, Forest Products Laboratory, 2024.
- Summary:
- "Gel filtration chromatography (GFC) is a form of aqueous liquid chromatography intended for separation of molecules based on effective molecular volume. With the advent of high-pressure liquid chromatography (HPLC) systems, liquid chromatography techniques have greatly improved. Further improvements have occurred with the development of more rigid and uniform stationary phase resins. GFC applications that employ modern liquid chromatography instrumentation and columns are often dubbed as "high-performance" (HPGFC). Given a need to study the process of enzymatic hemicellulose degradation, we determined that an HPGFC method would be beneficial to develop for use by the Forest Products Laboratory. In this report, we describe a laboratory method of HPGFC for the high-resolution separation of soluble hemicellulosic polysaccharides. Using this method, we can gain greater understanding of how xylan- and mannan-processing enzymes function."
- Notes:
- In scope of the U.S. Government Publishing Office Cataloging and Indexing Program (C&I) and Federal Depository Library Program (FDLP).
- "July 2024."
- Includes bibliographical references (page 7).
- Description based on online resource, PDF version; title from cover (FPL, viewed February 13, 2025).
- OCLC:
- 1446392746
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