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Cross-Species Investigation of Hypersomnia Genetics: A Role for Synaptic Adhesion Molecules Kyla Mace

Dissertations & Theses @ University of Pennsylvania Available online

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Format:
Book
Thesis/Dissertation
Author/Creator:
Mace, Kyla, author.
Contributor:
University of Pennsylvania. Pharmacology., degree granting institution.
Language:
English
Subjects (All):
Neurosciences.
Developmental biology.
Genetics.
0317.
0758.
0369.
Local Subjects:
Neurosciences.
Developmental biology.
Genetics.
0317.
0758.
0369.
Physical Description:
1 electronic resource (138 pages)
Contained In:
Dissertations Abstracts International 86-07B
Place of Publication:
Ann Arbor : ProQuest Dissertations and Theses, 2024
Language Note:
English
Summary:
Idiopathic hypersomnia (IH) is a poorly understood sleep disorder characterized by excessive daytime sleepiness despite normal nighttime sleep. Combining human genomics with behavioral and mechanistic studies in fish and flies, we uncover a role for beat-Ia/CADM2, synaptic adhesion molecules of the immunoglobulin superfamily, in excessive sleepiness. Neuronal knockdown of Drosophila beat-Ia results in sleepy flies and loss of the vertebrate ortholog of beat-Ia, CADM2, results in sleepy fish. We delineate a developmental function for beat-Ia in synaptic elaboration of neuropeptide F (NPF) neurites projecting to the suboesophageal zone (SEZ) of the fly brain. Brain connectome and experimental evidence demonstrate these NPF outputs synapse onto a subpopulation of SEZ GABAergic neurons to stabilize arousal. NPF is the Drosophila homolog of vertebrate neuropeptide Y (NPY), and an NPY receptor agonist restores sleep to normal levels in zebrafish lacking CADM2. These findings point towards NPY modulation as a treatment target for human hypersomnia
Notes:
Source: Dissertations Abstracts International, Volume: 86-07, Section: B.
Advisors: Kayser, Matthew Committee members: Kelz, Max; Sehgal, Amita; Raizen, David
Ph.D. University of Pennsylvania 2024
Local Notes:
School code: 0175
ISBN:
9798302182517
Access Restriction:
Restricted for use by site license

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