1 option
Niche Cytoskeletal Architecture Is Required for Proper Stem Cell Signaling and Oriented Division in the Drosophila Testis Gabriela Silva Vida
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Vida, Gabriela Silva, author.
- Language:
- English
- Subjects (All):
- Cellular biology.
- Neurosciences.
- Developmental biology.
- Genetics.
- 0379.
- 0317.
- 0369.
- 0758.
- Local Subjects:
- Cellular biology.
- Neurosciences.
- Developmental biology.
- Genetics.
- 0379.
- 0317.
- 0369.
- 0758.
- Physical Description:
- 1 electronic resource (93 pages)
- Contained In:
- Dissertations Abstracts International 86-07B
- Place of Publication:
- Ann Arbor : ProQuest Dissertations and Theses, 2024
- Language Note:
- English
- Summary:
- Stem cells are critical to repair and regenerate tissues, and often reside in a niche that controls their behavior. The Drosophila testis niche has been a paradigm for niche-stem cell interactions and is used here to address the cell biological features that maintain niche structure and function during its steady-state operation. Moreover, a snRNAseq dataset of the Drosophila testis can uncover to new factors that may be critical for these niche-stem cell interactions. Importantly, we report enrichment of the Myosin II (MyoII) and a key regulator of acto-myosin contractility (AMC), Rho Kinase (ROK), within the niche cell cortex at the interface with germline stem cells (GSCs). Compromising MyoII and ROK disrupts niche architecture, suggesting that AMC in niche cells is important to maintain the reproducible structure of this niche. Furthermore, defects in niche architecture cause changes in stem cell function. Our data suggest that the niche signals less robustly to adjacent germ cells, yet the disrupted structure permits increased numbers of cells to respond to the signal. Finally, compromising Myo II in niche cells leads to an increase in mis-oriented centrosomes in GSCs as well as defects in the centrosome orientation checkpoint. Ultimately, this work identifies a critical role for AMC-dependent maintenance of niche structure to ensure a proper complement of stem cells with correct execution of stem cell divisions
- Notes:
- Source: Dissertations Abstracts International, Volume: 86-07, Section: B.
- Advisors: DiNardo, Stephen Committee members: Sundaram, Meera; Bi, Erfei; Bashaw, Greg J.; Joyce, Eric F.
- Ph.D. University of Pennsylvania 2024
- Local Notes:
- School code: 0175
- ISBN:
- 9798302182630
- Access Restriction:
- Restricted for use by site license
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.