Organoids : models for development and disease / edited by Kursad Turksen.

Format:

Book

Contributor:

Turksen, Kursad, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2951.

Methods in molecular biology, 1940-6029 ; 2951

Language:

English

Subjects (All):

Tissue engineering--Laboratory manuals.

Tissue engineering.

Stem cells--Laboratory manuals.

Stem cells.

Cell organelles--Laboratory manuals.

Cell organelles.

Physical Description:

1 online resource (xii, 280 pages) : color illustrations.

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This volume explores the generation and characterization of organoids from different tissues, as these structures allow us to investigate questions about cell commitment and differentiation. Whether pursuing cell development or the genesis or treatment of diseases, organoid research, through the methods and techniques in this collection, aims to provide scientists with a way to regenerate different tissues or organs in their original form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Organoids: Models for Development and Disease serves as an ideal guide for researchers investigating this vital area of study"-- Springer Nature Link.

Contents:

Microfluidic chip fabrication for tumor cell 3d culture based on microwell arrays / Lanjie Lei

A simplified and robust immunofluorescence labeling method for complex 3d cell cultures : minimizing manipulation and maximizing data in whole-mount analysis of organoids, spheroids, and co-culture models / Gamze Demirel, Olgu E. Tok, and Ranan G. Aktas

A coculture system for modeling intestinal epithelial-fibroblast crosstalk / Rebecca F. Lee, Mei-Lan Li, Maria Figetakis, and Kaelyn Sumigray

Paraffin embedding and histological staining of intestinal organoids / Rachel Edens, Renata Rocha do Nascimento, Kristen A. Engevik, and Amy C. Engevik

Organoid culture of different intestinal segments from human and mouse / Yalong Wang, Ronghui Tan, and Ye-Guang Chen

Expansion and maturation of innate lymphoid cell precursors using human iPSC-derived intestinal organoids / Emma Højmose Kromann, Geraldine M. Jowett, and Joana F. Neves

Mouse small intestinal organoid cultures / Lei Chen and Xiaoting Xu

Establishing neural organoid cultures for investigating the effects of microgravity in low-earth orbit (LEO) / Nicolette A. Pirjanian, Kriti Kalpana, Ilya Kruglikov, Pinar Mesci, Jana Stoudemire, Paula Grisanti, Scott A. Noggle, Jeanne F. Loring, and Valentina Fossati

Production of neuroepithelial organoids from human-induced pluripotent stem cells for mimicking early neural tube development / Chunling Tang, Xinghui Wang, Eileen Gentleman, and Nicholas A. Kurniawan

Methods for generating self-organizing human patterned heart organoids using pluripotent stem cells / Brett Volmert and Aitor Aguirre

Generating neuroimmune assembloids using human induced pluripotent stem cell (iPSC)-derived cortical organoids and microglia / Kriti Kalpana, Chandrika Rao, Stefan Semrau, Bin Zhang, Scott Noggle, and Valentina Fossati

Generation of retinal organoids using human-induced pluripotent stem cells / Keith Theodore, Joel Alan Imventarza, Saleha Tahir, Bruna Lopes da Costa, and Peter M. J. Quinn

Transplantation of islet organoids into brown adipose tissue in a diabetic mouse model / Aixia Sun, Mankirat Singh, Manvir Bamrah, Wen Li, Aitor Aguirre, and Ping Wang

Spontaneous efficient differentiation of human pluripotent stem cells (hPSC) upon co-culture of hPSCs with human neonatal foreskin fibroblasts in 3d / Muhammad Nihad, Sudheer Shenoy P, and Bipasha Bose

Neuromuscular organoids to study spinal cord development and disease / Tobias Grass, Zeynep Dokuzluoglu, and Natalia Rodriguez-Muela

A robust human liver organoid model of hepatitis B virus infection / Bang M. Tran, Linda Earnest, Dustin J. Flanagan, Jean M. Moselen, Hoanh Tran, Joseph Torresi, and Elizabeth Vincan

Establishment of patient-derived organoids from colorectal cancer resection samples / Didem Seven, Szilvia Baron, and Henner F. Farin

Complex spheroids as an alternative : in vivo-like 3d model for investigating the impact of stromal cells on the radiation response of tumors / Hanna Sentek and Diana Klein

3D cell culture models as a platform for studying tumor progression, testing treatment responses, and discovering biomarkers / Peyda Korhan, Ezgi Bağırsakçı, Yasemin Öztemur Islakoğlu, and Neşe Atabey.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed November 11, 2025).

Other Format:

Print version: Organoids

ISBN:

9781071646823

1071646826

OCLC:

1531575945

Access Restriction:

Restricted for use by site license