Stem cell niche : methods and protocols / edited by Kursad Turksen.

Format:

Book

Contributor:

Turksen, Kursad, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2939.

Methods in molecular biology, 1940-6029 ; 2939

Language:

English

Subjects (All):

Stem cells--Laboratory manuals.

Stem cells.

Stem Cells.

Medical Subjects:

Stem Cells.

Physical Description:

1 online resource (xiii, 292 pages) : illustrations (some color).

Edition:

Third edition.

Place of Publication:

New York, NY : Humana Press, 2025.

Summary:

"This updated collection features protocols that reflect the continued expansion of stem cell niche research. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the second edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Niche: Methods and Protocols, Third Edition serves as an ideal guide for both experts and novices in the stem cell field"-- Srpinger Nature.

Contents:

Human skeletal muscle niche formation and analysis with spatial RNA sequencing / Ben Clock and Michael Hicks

Three-dimensional analysis of skeletal muscle stem cell niche following tissue clearing / Yoko Asakura, Smrithi Karthikeyan, and Atsushi Asakura

Efficient isolation and ex vivo differentiation of murine satellite cells from healthy and dystrophic muscle / Alessio A. Cusmano, Cordell A. VanGenderen, Tim O. Lorenz, Yafen Yang, and Natasha C. Chang

Isolation of mouse bone marrow niche cells for single-cell sequencing / Yaphet Bustos, Adrienne M. Dorrance, and Chinmayee Goda

Microwells as minimalistic niches to study heterotypic interactions of stromal and hematopoietic stem cells / Adrian Candelas, Thomas Bessy, Benoit Vianay, Manuel Théry, and Stephane Brunet

Detection of cellular senescence on murine muscle tissue sections by senescence-associated beta galactosidase staining / Wenxin Zhang and Tom H. Cheung

Light-sheet microscopy enables three-dimensional fluorescence imaging and live imaging of satellite cells on skeletal muscle fibers / Hira Asif Khan, Nick Van Hateren, and Anne-Gaëlle Borycki

Stem cell niche : iPSC-based assembloids for modeling human hematopoiesis / Madeline J. Caduc, Marcelo A.S. de Toledo, Steffen Koschmieder, and Simón Méndez-Ferrer

Oligopaint FISH in Drosophila testes / Romir Raj, Vedansh Patel, and Mayu Inaba

Evaluation of adult mouse brain neurogenic niche behavior culturing adult mice brain slice in vitro / Maria Isabel Alonso, Sonia Martínez-Páramo, Francisco Lamus, and Angel Gato

Isolation of murine neonatal and adult osteomacs to examine their role in the hematopoietic niche / Safa F. Mohamad and Melissa A. Kacena

Telocytes : detection, visualization, tissue dissociation, and tamoxifen-induction of transgenic mice / Marco Canella and Michal Shoshkes-Carmel

Co-colonization and co-culture of lung alveoli epithelial stem cells and their endothelial niche cells / Hiroyuki Hirai, Qian Chen, and Yuru Liu

Simplified quantification of progenitor zone size, an indicator of germ stem cell niche activity, in the nematode Caenorhabditis elegans / Sarah R. Fausett, Caroline A. Laury, Rachel E. Magallon, and Christian Braendle

Measurement of myonuclear accretion in vitro and in vivo / Lola Lessard, Audrey Saugues, Julien Gondin, Rémi Mounier, and Anita Kneppers

A simple method to dissect, orientate, and visualize the murine limbal stem cell niche with cornea and conjunctiva attached / Lamia Nureen and Nick Di Girolamo

Regeneration of volumetric muscle loss using MSCs encapsulated in PRP-derived fibrin microbeads / Şükran Şeker, Özge Lalegül-Ülker, Ayşe Eser Elçin, and Yaşar Murat Elçin

Standardizing chondrocyte isolation and articular cartilage decellularization : a versatile bioink for tissue engineering applications / Upasna Upadhyay, Kamma Srinivasulu, and Lakshmi Kiran Chelluri

Electrospinning approach for preparing nanostructured scaffolds for stem cell seeding and/or implantation in neurotrauma / Eldar F. Davletshin, Albert A. Rizvanov, and Yana O. Mukhamedshina

Development of a three-dimensional multicellular model of human neuroblastoma using Matrigel as an extracellular matrix analogue / Kristina V. Kitaeva, Valeriya V. Solovyeva, and Albert A. Rizvanov

Principles of indirect co-culture method using Transwell / Mohammad Rasouli and Fatemeh Safari

Upstream process protocol for MSCs isolated from different human-based tissue origins / Pelin Kaşıkcı, Cansu Özdemir, Begüm Coşar, Büşra Nigar Savran, Aysun Sarıkaya, Begüm Sargon, Alım Toprakkale, İrem Songür, Özlem Kandemir Seçgin, Pınar Akpınar Oktar, Elif Nazlı Çetindağ, Deniz Yurtsever Sarica, Serpil Taşdelen, Ustün Ezer, Ahmet Emin Kürekçi, and Günhan Gürman

Isolation and culturing of newborn rat nasal chondrocytes / Ekaterina I. Smirnova, Victoria A. Shestakova, Elena V. Isaeva, Anastas A. Kisel, Elena M. Yatsenko, Peter V. Shegay, Andrey D. Kaprin, Ilya D. Klabukov, and Denis S. Baranovskii.

Notes:

Includes index.

Online resource; title from PDF title page (SpringerLink, viewed August 12, 2025).

Other Format:

Print version: Stem cell niche.

ISBN:

9781071646113

1071646117

OCLC:

1531575149

Publisher Number:

CIPO000233392

Access Restriction:

Restricted for use by site license