Spermatogenesis : methods and protocols / edited by Prabhakara Poothi Reddi

Format:

Book

Contributor:

Reddi, Prabhakara Poothi, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 2954.

Methods in molecular biology, 1940-6029 ; 2954

Language:

English

Subjects (All):

Spermatogenesis--Laboratory manuals.

Spermatogenesis.

Physical Description:

1 online resource (xi, 296 pages) : illustrations

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

This volume presents a comprehensive collection of research methods at the cellular, molecular, and biochemical level to understand sperm production and function. Exploring spermatogenesis and testis biology, the book delves into epigenomics and transcriptomics, spermatogenic cell separation, identification of cell types and stages, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spermatogenesis: Methods and Protocols serves as an excellent reference for basic applied researchers in the field of spermatogenesis and male fertility working with laboratory models as well as domestic animals

Contents:

Profiling histone modifications in differentiating mouse spermatogonia with CUT\&Tag / Benjamin William Walters, Haoming Yu, Shubhangini Kataruka, and Bluma J. Lesch

Quantitative CUT\&Tag for epigenomic profiling of mouse germ cells / Mengwen Hu and Satoshi H. Namekawa

Spatial transcriptomic analyses of spermatogenesis / Ndifereke Uboh, Sean Vargas, Victoria D. Diaz, and Brian P. Hermann

Identification of RNA targets of highly conserved RNA binding proteins essential for animal sperm development / Min Zang, Liping Cheng, and Eugene Yujun Xu

Generation of organotypic testicular organoids from rat and pig primary cells in microwell culture / Anja Elsenhans, Nathalia de Lima e Martins Lara, Sadman Sakib, and Ina Dobrinski

Inhibiting aldehyde dehydrogenase function using WIN 18,446 to synchronize spermatogenesis / Shelby L. Havel and Michael D. Griswold

Individualization of testicular cells for single-cell subcellular immunocytochemistry and cell culture applications / Tracy M. Clement

Isolation of pig or rodent Sertoli cells for use in transplantation / Alexis R. Rodriguez, João Pedro Tôrres Guimarães, Anand Chakroborty, Rachel L. Babcock, Jonathan M. Miranda, Gurvinder Kaur, and Jannette M. Dufour

Density gradient-based separation of testicular cell types using a STA-PUT apparatus / Mirella L. Meyer-Ficca and Ralph G. Meyer

Antibody labeling of mouse spermatocyte chromosome spreads to study meiosis / Md Hasanur Alam and Huanyu Qiao

Transillumination-assisted microdissection for precise staging of seminiferous tubules in mice / Irene Infancy Joseph and Prabhakara Poothi Reddi

Immunofluorescence staining of the manchette and developing sperm flagella in mouse / Changmin Niu and Zhibing Zhang

Methods of mammalian in vitro sperm capacitation to study sperm physiology / Michal Zigo, Karl Kerns, and Peter Sutovsky

Analysis of motion characteristics and plasma membrane intactness (viability) in sperm from domestic animals / Camilo Hernández-Avilés

Methods to determine oxidative stress in spermatozoa / Jacinta H. Martin, Leah Calvert, Amanda L. Anderson, Tessa Lord, John E. Schjenken, Natalie A. Trigg, Geoffry N. De Iuliis, and Brett Nixon

Methods to evaluate DNA damage in spermatozoa / Jacinta H. Martin, Natalie A. Trigg, Leah Calvert, Amanda L. Anderson, Tessa Lord, John E. Schjenken, Shaun D. Roman, Geoffry N. De Iuliis, and Brett Nixon

Cre deleter mouse lines for conditional knockout of genes in testis cell types / T. Rajendra Kumar

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed March 4, 2026)

Other Format:

Print version: Spermatogenesis

ISBN:

9781071646984

1071646982

OCLC:

1527667652

Publisher Number:

CIPO000252790

Access Restriction:

Restricted for use by site license