Stem cells and aging : methods and protocols / edited by Kursad Turksen.

Format:

Book

Contributor:

Turksen, Kursad, editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in Molecular Biology, 1940-6029 ; 2960

Language:

English

Subjects (All):

Stem cells--Laboratory manuals.

Stem cells.

Aging--Molecular aspects--Laboratory manuals.

Aging.

Cells--Aging--Laboratory manuals.

Cells.

Cellular Senescence.

Stem Cells.

Cells--Aging.

Medical Subjects:

Cellular Senescence.

Stem Cells.

Physical Description:

1 online resource (xii, 306 pages) : illustrations.

Edition:

Third edition.

Place of Publication:

New York, NY : Humana Press, 2025.

Summary:

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Third Edition serves as an ideal guide for researchers working toward furthering our understanding of the aging process.

Contents:

Protocols to assess the aging of human mesenchymal stem cells / Merdiye Mavis and Nedime Serakinci

Analyzing muscle stem cell function ex vivo / Julia von Maltzahn

Tubular aggregates as a marker of aging in skeletal muscle / Felipe Tadeu Galante Rocha de Vasconcelos, Brandow Willy Souza, Lucas Santos Souza, and Mariz Vainzof

Fusion of human synovium-derived mesenchymal stem/stromal cells with primary human chondrocytes using the modified adherence method (MAM) / Tilen Burnik, Janja Zupan, Matjaž Jeras, and Maša Kandušer

Multiplex PCR for the rapid diagnosis of myeloproliferative neoplasms / Laura Semenuk, Alissa Palazzolo, Kristen McCambridge, Henry Wong, Michael J. Rauh, Harriet Feilotter, and Graeme Quest

Ex vivo expansion of cord blood hematopoietic stem and progenitor cells / Pinar Yurdakul-Mesutoglu, Hasan Yalim Akin, and Zeynep Gunaydin

Evaluation of anti-aging agents using the D-galactose-induced accelerated aging model / Serdar Bora Bayraktaroğlu, Raife Dilek Turan, Neslihan Pakize Taşlı, and Fikrettin Şahin

Methods to detect and compare cellular and mitochondrial changes in senescent and healthy mesenchymal stem cells / Afshin Samiminemati, Mohd Shahzaib, Claudia Moriello, Nicola Alessio, Domenico Aprile, Tiziana Squillaro, Giovanni Di Bernardo, and Umberto Galderisi

Transfer of mitochondria from healthy stem cells to injured cells in stroke with retinal impairments / Napasiri Putthanbut, Jea-Young Lee, and Cesario V. Borlongan

A protocol for detecting DNA methylation changes at CpG sites of stemness-related genes in aging stem cells / Mahshid Hodjat, Seyed Mojtaba Daghighi, and Mohammad Abdollahi

Robust expansion of hematopoietic stem cells ex vivo using small molecule cocktails / Woratree Kaewsakulthong, Ajay Ratan Pasala, Justine Hanotaux, Tanvir Hasan, and Harinad B. Maganti

A genome-wide CRISPR/Cas9 screen identifies regulatory genes for stem cell aging / [No author listed]

Immunocompetent brain organoids with microglia allow advanced aging research / Raiane Oliveira Ferreira, Amanda Faria Assoni, Monize Valéria Ramos da Silva, Letícia Alves da Rocha, Mateus Vidigal de Castro, Débora Bertola, and Mayana Zatz

Quality control in human adipose-derived stromal/stem cells and tissue engineering fat models for aging studies / Katie Hamel, Jordan Robinson, Emma Rogers, Haley Lassiter, Trivia Frazier, and Cecilia Sanchez

Mesenchymal stromal cell (MSC) isolation and induction of acute and replicative senescence / Afshin Samiminemati, Mohd Shahzaib, Claudia Moriello, Nicola Alessio, Domenico Aprile, Tiziana Squillaro, Giovanni Di Bernardo, and Umberto Galderisi

Delivery of stem cell-rejuvenating compounds via subcutaneous osmotic pumps / Sen Zhang and Sandra Pinho

Isolation procedure for rat pancreatic ductal cells / Nazli Karimi and Gulbahar Boyuk Ozcan

Polychromatic flow cytometry to identify rare aged hematopoietic stem cell subpopulations / Natalia Skinder, Christina Pitsillidou, Alessandra Roberto, and Gerald de Haan

Isolation of human hematopoietic stem cells from an apheresis sample / Pınar Siyah and Fatih Kocabaş

Quantifying muscle regeneration : activated muscle satellite cells and new regenerated myofibers in chronic and acute degeneration models / Antonio Fernando Ribeiro Junior, Brandow Willy Souza, and Mariz Vainzof

Identification of de novo dividing stem cells / Dmitry I. Maltsev and Oleg V. Podgorny

Rat ductal cell-derived differentiation into islet-like cells / Nazli Karimi and Gülbahar Boyuk Ozcan,

Notes:

Includes index.

Online resource; title from PDF title page (SpringerLink, viewed August 4, 2025).

Other Format:

Print version: Stem cells and aging

ISBN:

9781071647189

1071647180

OCLC:

1530444712

Publisher Number:

CIPO000252794

Access Restriction:

Restricted for use by site license