Epitope mapping protocols edited by Johan Rockberg, Johan Nilvebrant, Magdalena Malm and Niklas Berndt Thalén

Format:

Book

Contributor:

Rockberg, Johan, editor.

Nilvebrant, Johan, editor.

Malm, Magdalena, editor.

Thalén, Niklas Berndt, editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology 1940-6029

Language:

English

Subjects (All):

Antigenic determinants--Research--Methodology.

Antigenic determinants.

Antigen-antibody reactions--Research--Methodology.

Antigen-antibody reactions.

Immunospecificity.

Epitopes.

Medical Subjects:

Epitopes.

Physical Description:

1 online resource

Edition:

Fourth edition

Place of Publication:

Springer US 2025

New York, NY Humana Press 2025

Summary:

This detailed new edition explores methodologies for the characterization of antibody-antigen interactions tailored to different applications and requirements. Beginning with an overview of key concepts and definitions related to antibody epitopes, the book continues with sections on structural biology methods, display technologies to analyze libraries of proteins or protein arrays, techniques based on protein labeling, as well as computational approaches and binding assays that rely on binding interactions or competition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Epitope Mapping Protocols, Fourth Edition serves as an ideal resource for scientists from a wide range of fields involved in critical antibody research. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Contents:

An Overview of Epitopes and Methods for Antibody Epitope Mapping

Antibody-Antigen Epitope Mapping by X-Ray Crystallography

Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations

Cryo-Electron Microscopy-Based Epitope Mapping

Epitope Mapping Using Yeast Display and Next Generation Sequencing

Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing

High-Density Antigen Microarrays for the Assessment of Antibody Selectivity

Mammalian Cell Display with Automated Oligo Design and Library Assembly Allows for Rapid Residue Level Conformational Epitope Mapping

Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries

Mapping of Antibody Structural Epitopes Using Bacterial Cell Surface Display of Folded Domains

Highly Multiplexed Immunoassays for the Validation of Anti-GPCR Antibodies

Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers

Genetic Code Expansion to Enable Site-Specific Post-Translational Peptide Epitope Tagging of G Protein-Coupled Receptors in Live Cells

Multiplexed Epitope Mapping of HIV-1 Neutralizing Antibodies by Coupling Chemical Labeling of Cysteines to Deep Sequencing

Predicting Epitopes of Synthetic Binding Proteins Using Protein Structure Prediction and Protein-Protein Docking

Structure-Based Epitope Profiling with the Structural Profiling of Antibodies to Cluster by Epitope 2 (SPACE2) Algorithm

Binding Kinetics and Epitope Binning Using Surface Plasmon Resonance

High-Throughput Epitope Binning Analysis of Human Monoclonal Antibodies to Assess Epitope Diversity Using the Octet Bio-Layer Interferometry Platform

Competitive Epitope Binning Using HT-SPR

Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors

B Cell Epitope Mapping by High Density Competition ELISAs

Notes:

Includes index

Online resource; title from PDF title page (SpringerLink, viewed July 10, 2025)

Other Format:

Print version :

ISBN:

9781071645918

1071645919

OCLC:

1527614412

Publisher Number:

CIPO000228365

Access Restriction:

Restricted for use by site license