Protein arrays : methods and protocols / edited by Cristina Ruiz-Romero, Lucía Lourido, Valentina Calamia.

Format:

Book

Contributor:

Ruiz-Romero, Cristina, editor.

Lourido, Lucía, editor.

Calamia, Valentina, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 2929.

Methods in Molecular Biology, 1940-6029 ; 2929

Language:

English

Subjects (All):

Protein microarrays--Laboratory manuals.

Protein microarrays.

Physical Description:

1 online resource (xi, 240 pages) : illustrations (some color)

Place of Publication:

New York, NY : Humana Press, [2025]

System Details:

text file PDF

Summary:

"This volume discusses the latest methods and applications of protein microarrays and helps researchers harness its power in their work. The chapters in this book explore protein microarray’s development and fabrication, strategies for their data analysis, and wide-reaching applications in biology and disease research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Arrays: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, laboratory scientists, and industry professionals who wish to learn more about protein microarray technology"-- Springer Nature Link.

Contents:

Printing high-density human protein fragments on epoxy-treated slides / Eni Andersson, Jennie Olofsson, Lovisa Skoglund, Ceke Hellström, and Ronald Sjöberg

Building reversed-phase microarrays on low-cost biosensing chips : Application to multiplexed detection of food allergies / Luis Antonio Tortajada-Genaro

Bead-based suspension array using peptides as antigens to capture and identify serum antibodies / Fimme J. van der Wal, Marloes Heijne, and René P. Achterberg

On-array citrullination of protein microarrays / Jacob Skallerup, Thomas B. G. Poulsen, and Allan Stensballe

High-throughput post-translational modification analyses by reverse phase protein array / Zhongcheng Shi, Michael Hoang Nguyen, Yuan Yao, and Shixia Huang

Optimization of serum and microarray concentrations for a semiquantitative multiplex indirect immunoassay / Patricia Quaranta, Rocío Paz-González, Selva Riva-Mendoza, Pablo Domínguez-Guerrero, Francisco J. Blanco García, Cristina Ruiz-Romero, Lucía Lourido, and Valentina Calamia

Protein microarray analysis with GenePix / Rodrigo Barderas, Ana Montero-Calle, and Pablo San Segundo-Acosta

Evaluating cross-reactivity in custom-made multiplex bead-based antibody microarrays : A methodological approach / Rocío Paz-González, Patricia Quaranta, Selva Riva-Mendoza, Pablo Domínguez-Guerrero, Francisco J. Blanco García, Cristina Ruiz-Romero, Valentina Calamia, and Lucía Lourido

SomaScan bioinformatics : Normalization, quality control, and assessment of pre-analytical variation / Julián Candia

Proteomics by qPCR using the proximity extension assay (PEA) / Julio Manuel Martínez-Moreno, Adrián Llamas-Urbano, Nuria Barbarroja, and Carlos Pérez-Sánchez

Multi-array technology for secreted cytokine profiling in a nano-aerosol exposed 3D human alveolar cell model / An Jacobs and Inge Nelissen

Use of humanized NFAT-Dsred RBL reporter for detection of allergen-specific IgE sensitization in human serum in 96-well microarray format / Daniel Wan, Marcos J.C. Alcocer, and Franco H. Falcone

A competitive ligand-binding assay for simultaneous detection of neutralizing antibodies to both arms of a bispecific drug / Vitaly Ablamunits, Soma Basak, Rosemary Lawrence-Henderson, Teresa M. Caiazzo, and John Kamerud

Alternative matrices for protein biomarker analysis by qPCR using proximity extension assay (PEA) / Adrián Llamas-Urbano, Julio Manuel Martínez-Moreno, Carlos Pérez-Sánchez, and Nuria Barbarroja

Cytokine antibody microarray-based proteomic strategies for characterizing the dysregulation of cytokines disease-specific / Rodrigo Barderas, Pablo San Segundo-Acosta, and Ana Montero-Calle

Unravelling immunogenic cell death mechanisms by functional proteomics arrays / Ana Nuño-Soriano, Carlota Arias-Hidalgo, Pablo Juanes-Velasco, Rafael Góngora, Ángela-Patricia Hernández, and Manuel Fuentes

Combination of antibody arrays to characterize the effect of neuropathological stimulus in human olfactory neuroepithelial cells / Mercedes Lachén-Montes, Elena Anaya-Cubero, Paz Cartas-Cejudo, Leire Extramiana, Joaquín Fernández-Irigoyen, and Enrique Santamaría.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed March 24, 2026).

Other Format:

Print version: Protein arrays

ISBN:

9781071645956

1071645951

OCLC:

1527666839

Publisher Number:

CIPO000240701

Access Restriction:

Restricted for use by site license