CRISPR-Cas systems for RNA and genome editing. Part A / edited by Ailong Ke, Weixin Tang.

Format:

Book

Contributor:

Ke, Ailong, editor.

Tang, Weixin, editor.

Series:

Methods in enzymology ; v. 712.

Methods in enzymology, 0076-6879 ; volume 712

Language:

English

Subjects (All):

Gene editing.

RNA editing.

CRISPR-associated protein 9.

CRISPR (Genetics).

Physical Description:

xxiv, 551 pages : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, an imprint of Elsevier, 2025.

Contents:

Measuring double-strand break repair events in mammalian cells with multi-target CRISPR / Alberto Martin-Gonzalez, Adam T. Rybczynski, Roger S. Zou, and Taekjip Ha

General and robust sample preparation strategies for cryo-EM studies of CRISPR-Cas9 and Cas12 enzymes / Satoshi N. Omura and Osamu Nureki

Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies / Grace N. Hibshman and David W. Taylor

Biochemical reconstitution of a type I-B CRISPR-associated transposon / Shukun Wang and Leifu Chang

Determining the biochemical function of type IV CRISPR ribonucleoprotein complexes and accessory proteins / Andrew A. Williams, Olivine Redman, Hannah Domgaard, Matthew J. Armbrust, and Ryan N. Jackson

Analysis of metal-dependent DNA nicking activities by Cas endonucleases / Giang T. Nguyen, Akshara Raju, and Dipali G. Sashital

Purification and in vivo, cell-free, and in vitro characterization of CRISPR-Cas12a2 / Friso T. Schut, Thomson Hallmark, Oleg Dmytrenko, Ryan N. Jackson, and Chase L. Beisel

Experimental strategy for characterization of novel TnpB orthologs / Gytis Druteika, Tautvydas Karvelis, and Virginijus Šikšnys

Hydrolytic endonucleolytic ribozyme (HYER): Systematic identification, characterization and potential application in nucleic acid manipulation / Zi-Xian Liu and Jun-Jie Gogo Liu

Methods for Cas13a expression and purification for use in CRISPR diagnostics / Her Siang Chai, Rebecca S. Bamert, and Gavin J. Knott

Using CRISPR for viral nucleic acid detection / Maaike S. Grimm and Cameron Myhrvold

Preparation of high-purity RNPs of CRISPR-based DNA base editors / Mitchell J. McAndrew, Madeleine B. King, and Audrone Lapinaite

Bacterial directed evolution of CRISPR base editors / Reilly Q. Mach and Shannon M. Miller

Genome editing with programmable base editors in human cells / Nicola R.B. Osgood, Natalie M. Zawalick, Courtney B. Sawyer, Quinn T. Cowan, Sifeng Gu, S.J. Mawson, Brodie L. Ranzau, Lehan Li, Melissa Gymrek, Alon Goren, and Alexis C. Komor

Prime editing in bacteria with BacPE / Hongyuan Zhang and Quanjiang Ji

A quick guide to evaluating prime editing efficiency in mammalian cells / Chengfang Liu, Sifan Cheng, Junjie Zhu, Lina Zhou, and Jia Chen

Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries / Samuel I. Gould and Francisco J. Sánchez-Rivera

Cloning and validating systems for high throughput molecular recording / Anqi Zhao and Michelle M. Chan

Delivery of genome editors with engineered virus-like particles / Christopher Lu, Yuanhang Li, Jacob Ryan Cummings, and Samagya Banskota

CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells / Rithu K. Pattali, Izaiah J. Ornelas, Carolyn D. Nguyen, Da Xu, Nikita S. Divekar, and James K. Nuñez.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780443317521

0443317526

OCLC:

1517976633

Publisher Number:

CIPO000180486