3D Bioprinting in Cancer Applications.

Format:

Book

Author/Creator:

Dilnawaz, Fahima.

Contributor:

Sharma, Tripti.

Sahoo, Sanjeeb K.

Senapati, Shantibhusan.

Language:

English

Physical Description:

1 online resource (394 pages)

Edition:

1st ed.

Place of Publication:

Chantilly : Elsevier Science & Technology, 2025.

Summary:

3D bioprinting in Cancer Applications is an all-encompassing guide covering both fundamentals concerning biofabrication principles and intricate protocols for precise construction of biomimetric 3D systems supporting advanced cell culture development that faithfully recapitulates human disease hallmarks, with a special focus on cancer.

Contents:

Front Cover

3D Bioprinting in Cancer Applications

Copyright

Contents

Contributors

Preface

1 - Hydrogel-microfluidic devices for 3D cell culture

1. Introduction

2. Fundamental principles and theories

3. Biocompatibility

4. Swelling and porosity

5. Fluid control with integration of channels

6. Biological compatibility

7. Methodology

8. Applications

9. Key features

10. Future prospects

11. Case studies

12. Conclusion

References

2 - Hydrogel-patterning technique for 3D cell culture

2. Two-dimensional (2D) cell culture and their associated drawbacks

3D cell culture technique

4. Hydrogel

4.1 Scaffold hydrogel biomaterials/materials for 3D cell culture

4.1.1 Natural polymeric hydrogel

4.1.2 Synthetic polymeric hydrogel

4.2 Different technique-hydrogel

4.3 Disease-based hydrogel techniques

4.3.1 Hydrogel technique for 3D culture of cartilage tissue

5. Conclusion

3 - Scaffold-based 3D cellular models for cancer research: Advances in tissue engineering and bioprinting

2. Fundamentals of scaffold-based 3D cellular models

2.1 Definition of scaffolds in tissue engineering

2.2 Types of scaffolds used in 3D cellular models

2.2.1 Natural scaffolds

2.2.2 Synthetic scaffolds

2.2.3 Composite scaffolds

2.2.4 Decellularized scaffolds

2.2.5 Hydrogel scaffolds

2.2.6 Nanofibrous scaffolds

2.2.7 Micropatterned scaffolds

2.3 Criteria for scaffold selection

2.3.1 Safety standards

2.3.2 Load capacity and strength

2.3.3 Adaptability and flexibility

2.3.4 Site conditions

2.3.5 Access requirements

2.3.6 Cost considerations

2.3.7 Manufacturer reputation and support

2.4 Challenges and limitations in scaffold design.

3. Principles of cancer tissue engineering

3.1 Characteristics of cancer cells and tumor microenvironment

3.1.1 Cancer cells

3.1.2 Uncontrolled growth and division

3.1.3 Genetic instability

3.1.4 Resistance to cell death

3.1.5 Ability to invade and metastasize

3.1.6 Angiogenesis

3.1.7 Metabolic reprogramming

3.1.8 Immune evasion

3.1.9 Tumor microenvironment

3.1.10 Immune cells

3.1.11 Stromal cells

3.1.12 Extracellular matrix

3.1.13 Signaling molecules

3.2 Importance of recapitulating tumor physiology in vitro

3.3 Role of 3D models in studying cancer progression and treatment response

4. Scaffold fabrication techniques

4.1 Overview of scaffold fabrication methods

4.1.1 Electrospinning

4.1.2 D printing

4.1.3 Self-assembly

4.2 Case studies highlighting innovative scaffold fabrication approaches

4.2.1 Case study 1: 3D bioprinting of customized scaffolds

4.2.2 Case study 2: Electrospinning of nanofibrous scaffolds

4.2.3 Case study 3: Self-assembling peptide scaffolds for cartilage regeneration

5. Cell-scaffold interactions

5.1 Cell adhesion, migration, and proliferation on scaffolds

5.1.1 Cell adhesion

5.1.2 Cell migration

5.1.3 Cell proliferation

5.2 Influence of scaffold properties on cellular behavior

5.2.1 Mechanical properties

5.2.2 Surface topography

5.2.3 Porosity

5.2.4 Bioactive cues

5.3 Signaling pathways involved in cell-scaffold interactions

5.3.1 Extracellular matrix signaling

5.3.2 Growth factor signaling

5.3.3 Wnt signaling

5.3.4 Notch signaling

5.3.5 Hedgehog signaling

5.3.6 Mechanosensitive signaling

6. Applications of 3D cellular models scaffold in cancer research

6.1 Drug screening and personalized medicine

6.2 Mechanistic studies of cancer progression and metastasis.

6.3 Evaluation of therapeutic interventions and biomaterials

7. Challenges and future directions

8. Conclusion

4 - Bioinformatics approaches for the validation of functional analysis of 3D cancer models

2. Bioinformatics tools and databases in cancer research

3. Bioinformatic approaches to understanding cancer biology

3.1 Systems biology approaches

3.2 Multiomics-based approaches

3.3 Pathway analysis-based approaches

4. Bioinformatics approaches toward validation of functional analysis of 3D cancer models

4.1 3D preclinical models for cancer research

4.2 Sequencing validation of patient-derived 3D cancer models for drug efficacy screens

4.3 3D models of cancer, single-cell profiling, and genomic screens as scalable discovery platforms

4.4 Single-cell profiling of tumor-microenvironments in 3D cancer models

4.5 3D cancer models for neoantigen discovery

4.6 Bioinformatics in case studies involving 3D cancer models

5. Conclusions

5 - Multidimensional liquid patterning for 3D microtissue engineering

2. Liquid trap phenomenon in the 3D micromesh structure

Tissue engineering of 3D organotypic microtissues

3.1 Acoustic assembly

3.2 Organoid generation

4. Organ-on-chip

4.1 Lung-on-chip

4.2 Brain-on-chip

4.3 Heart-on-chip

4.4 Liver-on-chip

4.5 Kidney-on-chip

4.6 Skin-on-chip

4.7 Gut-on-chip

4.8 Multiple organs-on-chip

4.9 3D bioprinting of vascularized tissues

5. 3D micromesh-based hybrid bioprinting

5.1 Photomask-based techniques

5.2 Micromold-based techniques

5.3 Rapid prototyping-based techniques

5.3.1 Photoliable rapid prototyping strategies

5.3.2 High-temperature rapid prototyping strategies

6. Application of microfabrication in 3D tissue engineering.

6.1 Cell-material and cell-cell interaction control

6.2 Vascularization of the 3D engineered tissues

6.3 Guiding the fate of stem cells with microengineered platforms

6.4 Combining surface modification and three-dimensional (3D) fabrication

6.5 3D photofabrication for drug delivery and tissue engineering

6.6 3D cell patterning for tissue engineering using magnetic scaffolds

7. Cell patterning and tissue engineering applications

7.1 Organ/disease modeling

7.2 Pharmacology and vascular targeted carriers

7.3 Personalized therapy and diagnosis

7.4 Heart valve reconstruction

7.5 3D bioprinting in studying bone

8. Future perspective and challenges

6 - Injection-molded plastic array three-dimensional universal culture platform for various biological applications

2. Promising characteristics of U-IMPACT for biological research

3. Fabrication procedure of the U-IMPACT platform

3.1 Core architect and mold fabrication for U-IMPACT platform

3.2 Material selection and injection modeling processing

3.3 Cooling and solidification

3.4 Mold release, demolding, and postprocessing

3.5 Packaging and sterilization of the unit

4. Biological applications of the U-IMPACT platform

4.1 U-IMPACT in tissue engineering

4.1.1 Scaffold architecture

4.1.2 Cell seeding and culture

4.1.3 Cell-cell interactions

4.1.4 Mechanical stimulation

4.1.5 Vascularization

4.2 U-IMPACT in drug screening

4.2.1 High-throughput screening

4.2.2 Improvement predictivity

4.2.3 Disease modeling

4.2.4 Personalized medicine

4.2.5 Mechanism of action studies

4.3 U-IMPACT in cancer research

4.3.1 Tumor modeling

4.3.2 Anticancer drug screening

4.3.3 Microenvironment studies

4.3.4 Metastasis research

4.3.5 Personalized medicine.

4.4 U-IMPACT in neuroscience research

4.4.1 Neuronal development and plasticity

4.4.2 Neuronal network formation

4.4.3 Electrophysiological studies

4.4.4 Disease modeling

4.4.5 Drug screening

4.5 U-IMPACT in microbiology and antibacterial drug discovery

4.5.1 Microbial culture

4.5.2 Biofilm formation analyses

4.5.3 Antibacterial drug screening

4.5.4 Mechanism of action studies

4.5.5 Synergistic interaction

5. Clinical advantages and challenges of U-IMPACT platforms

6. Conclusion

Abbreviations

Conflicting interest statement

7 - 3D cell-culture microfluidic devices for tumor microenvironment biomarker profiling

2. Conventional approaches for the construction of in vitro 3D cancer models

3. Bioprinting: A novel strategy for building a biomimetic cancer model

3.1 Inkjet bioprinting

3.2 Extrusion-based bioprinting

3.3 Light-assisted bioprinting

4. Characteristics for generating 3D bioprinting tumor model

4.1 Biomaterials for 3D-printed cancer models

4.2 Cells for 3D-printed cancer models

4.3 Tumor vasculature and immune environment

4.4 3D-printed models as drug screening platforms

5. Case studies of bioprinting cancer

5.1 Bioprinting lung cancer model

5.2 Glioblastoma

5.3 Skin

5.4 Colorectal

5.5 Ovarian and cervical cancer

6. Advantages of 3D printed cancer models

7. Challenges of 3D printed cancer models

8 - 3D bioprinting for recapitulation of tumor microenvironment

1. Understanding the complexity of cancer and the tumor microenvironment

1.1 Complexity and heterogeneity of the tumor microenvironment

1.2 Evolution of in vitro models for studying the tumor microenvironment

2. Exploration of 3D bioprinting in cancer modeling.

2.1 Advantages of 3D bioprinting for recapitulation of tumor microenvironment.

Notes:

Description based on publisher supplied metadata and other sources.

ISBN:

0-443-26519-4

OCLC:

1523291819