Molecular virology : methods and protocols / edited by Chunfu Zheng.

Format:

Book

Contributor:

Zheng, Chunfu, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 v. 2940.

Methods in molecular biology, 1940-6029 ; 2940

Language:

English

Subjects (All):

Molecular virology--Methodology.

Molecular virology.

Physical Description:

1 online resource (xv, 401 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

This detailed book presents classical and cutting-edge techniques in molecular virology research. The volume begins with guidelines for investigating viral structure, proliferation processes, and virulence factors, followed by various approaches to identify key elements and molecular signaling pathways in virus-infected host cells that regulate virus-host interactions. Additional chapters explore methods for studying autophagy, post-translational modifications of virus and host proteins, epigenetic factors in anti-viral immune responses, and viral epidemiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Virology: Methods and Protocols serves as an ideal guide to this vitally important area of study.

Contents:

Investigation of Protein Fatty Acylation in Virus-Host Interaction

Determination of Protein Ubiquitination after Protein Denaturation in Virus-Host Interactions

Viral Circuit Tracing in Biomedical Research: Methods, Protocols, and Applications

Analysis of the Virus-Host Protein-Protein Interaction via Co-Immunoprecipitation (Co-IP)

Tandem Affinity Purification of Virus-Host Protein Complexes via the Strep/FLAG (SF)-TAP Tag

Applying 3D Genome Technology in Virology Research

In Vitro Liquid-Liquid Phase Separation Assay for Viral Proteins

Computational Analysis of Virus-Host Interactomes

Application of Single-Cell RNA Sequencing in Investigating Virus-Host Interactions

CRISPR-Mediated Viral Gene Knock-In for Studying Viral-Host Interactions

High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology

Pull-Down Techniques for Determining Virus-Host Protein Interactions

Application of Organoids to Investigate Virus-Host Interactions

Identification of Virus-Host Protein Interactions via Proteomic Techniques

Engineering of Vesicular Stomatitis Virus (VSV) for Modulating Cell Death and Antitumor Immunity

3D Tissue Culture Model for Virology Studies

Detection of Protein Interactions During Viral Infection by Bimolecular Fluorescence Complementation

Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity

The Application of HSV-1 in Investigating Neurological Diseases

Strategy and Application of Viral Potency Assays in Cell and Gene Therapy

Chromatin Immunoprecipitation (ChIP)-Quantitative PCR (qPCR) Analysis of Viral Genome-Host Protein Interactions

The Application of Base Editing Technology to Investigate Virus-Host Interactions and Antiviral Therapeutic Strategies

Zebrafish as a Model for Studying Virus-Host Interactions

Screening for Host Proteins with Pro- and Antiviral Activity via High-Throughput CRISPR

Isotope Labeling Techniques for Virus-Infected Cells

Reverse Genetics-Based Methodology for Molecular Virology Study

RBD Amplicon Sequencing and Evolutionary Analysis of SARS-CoV-2 Variants from Wastewater Samples

RNA Binding Protein Immunoprecipitation 1 (RIP) Assay to Investigate the Interactions Between Viral RNA and Host Proteins

Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions

Electrophoretic Mobility Shift Assay for Identification of the Interaction Between a Viral Protein and DNA

Applying Phage Display Technology to Obtain Specific Peptides Blocking Host-Virus Interactions

Single Particle Cryo-EM to Investigate Nonenveloped Virus-Like Particles

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) to Identify DNA Motifs Mirroring Chromosome Targets of the Hypothetical Transcription Factor NLRP3

Detection of Protein Interactions and Dynamics During Viral Infection via Fluorescence Resonance Energy Transfer.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed June 17, 2025).

Other Format:

Print version: Molecular virology

ISBN:

9781071646151

107164615X

OCLC:

1523878015

Publisher Number:

CIPO000233393

CIPO000233394

Access Restriction:

Restricted for use by site license