Tick-borne viruses : methods and protocols / edited by Saravanan Thangamani.

Format:

Book

Contributor:

Thangamani, Saravanan, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 2936.

Methods in molecular biology, 1940-6029 ; 2936

Language:

English

Subjects (All):

Tick-borne diseases.

Tick-Borne Diseases.

Medical Subjects:

Tick-Borne Diseases.

Genre:

Laboratory manuals.

Physical Description:

1 online resource : illustrations (some color).

Place of Publication:

New York : Humana Press, [2025]

Summary:

"This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus’ biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues. Cutting-edge and thorough, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs"-- Provided by publisher.

Contents:

Tick cell culture methods / Xin-Ru Wang, Benjamin Cull

Viral isolation and culture from field-collected ticks : In vitro / Charles E. Hart, Saravanan Thangamani

Virus isolation from field-collected ticks : In vivo / Jessica Crooker, Dakota N. Paine, Saravanan Thangamani

Generation of ticks infected with tick-borne encephalitis virus / Pavlína Bartíková, Iveta Štibrániová, Mária Kazimírová

Generation of virus-infected ticks : Synchronous infection and feeding on viremic animals / Erin S. Reynolds, Saravanan Thangamani

Human skin ex vivo culture for tick-borne virus infection studies / Dakota N. Paine, Jessica Crooker, Saravanan Thangamani

Colorado tick fever virus : Quantification, propagation, storage, and detection / Stephanie Beane, Megan Burch, Jeremy Bechelli

Isolation and quantification of

Dabie bandavirus / Diana Fernández, Patricia V. Aguilar

Immunohistochemical assays to visualize tick-borne flaviviruses at the tick feeding site / Rodrigo I. Santos, Meghan E. Hermance

Immunofluorescence assays to visualize tick-borne flaviviruses in mouse skin or brain / Rodrigo I. Santos, Meghan E. Hermance

RNA in situ hybridization to visualize tick-borne viruses / Allen T. Esterly, Erin S. Reynolds, Charles E. Hart, Saravanan Thangamani

Quantification of tick-borne flaviviruses using focus forming assays / Allen T. Esterly, Erin S. Reynolds, Saravanan Thangamani

Quantification of neutralizing antibodies of tick-borne viruses using focus neutralization assays / Allen T. Esterly, Saravanan Thangamani

Methods for evaluation of antivirals and neutralizing antibodies against tick-borne encephalitis virus / Ludek Eyer, Pavel Svoboda, Martin Palus, Jiri Salat, Daniel Růžek

Exploring tick-borne encephalitis virus-host cell interactions using electron tomography : Methodologies and protocols / Marie Vancová, Tomáš Bílý, Martin Palus, Daniel Růžek.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed October 6, 2025)

Other Format:

Print version: Tick-borne viruses

ISBN:

9781071645871

1071645870

OCLC:

1522004898

Publisher Number:

CIPO000233390

CIPO000233389

Access Restriction:

Restricted for use by site license