Fundamentals of fluorescence microscopy exploring life with light Partha Pratim Mondal, Alberto Diaspro

Format:

Book

Author/Creator:

Mondal, Partha Pratim, author.

Diaspro, Alberto, 1959- author.

Language:

English

Subjects (All):

Fluorescence microscopy.

Microscopy, Fluorescence.

fluorescence microscopy.

Medical Subjects:

Microscopy, Fluorescence.

Physical Description:

1 online resource

Place of Publication:

Dordrecht Springer 2014

Summary:

This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging

Contents:

Ray Optics, Wave Optics and Imaging System Designing

Basics of Electromagnetic Theory for Fluorescence Microscopy

Electric Field Effects in Optical-Microscopy Systems

Quantum Description of Radiation Field and Optical Microscopy

Molecular Physics of Fluorescence Markers

Basics of Fluorescence and Photophysics

General Fluorescence Imaging Techniques

Multiphoton Fluorescence Microscopy

Super Resolution Fluorescence Microscopy

Image Reconstruction Methodologies for Fluorescence Microscopy

Future Prospective of Fluorescence Microscopy

Notes:

Includes bibliographical references

Online resource; title from PDF title page (SpringerLink, viewed December 16, 2013)

Other Format:

Printed edition:

ISBN:

9789400775459

9400775458

940077544X

9789400775442

OCLC:

872568712

Access Restriction:

Restricted for use by site license