Cancer immunosurveillance methods and protocols edited by Alejandro López-Soto and Alicia R. Folgueras

Format:

Book

Contributor:

Lopez-Soto, Alejandro, editor.

Folgueras, Alicia R., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology 1940-6029

Language:

English

Subjects (All):

Cancer--Immunological aspects--Laboratory manuals.

Cancer.

Cancer--Immunological aspects.

Cancer--Immunodiagnosis.

Physical Description:

1 online resource

Edition:

Second edition

Place of Publication:

Springer US 2025

New York, NY Humana Press 2025

Summary:

This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of immune cells with antitumor properties, bioinformatic approaches of interest for cancer immunology research, techniques for performing epigenetic studies, as well as methods related to translational research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cancer Immunosurveillance: Methods and Protocols, Second Edition serves as a valuable resource for scientists seeking inspiration for new ideas and research in the field of tumor immunology

Contents:

Characterization of Bone Marrow-Derived Immune Compartment in Murine Brain Metastases by Spectral Flow Cytometry

Multicolor Flow Cytometry for Immune Characterization of Omental Metastasis in Peritoneal Carcinomatosis Mice Models

Application of Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq) to Precisely Dissect the Brain Tumor Immune Microenvironment

Magnetic Bead Based Isolation of CD8+ T Cells from the Mouse Spleen

Phosphoflow: Evaluation of Protein Phosphorylation Status by Cytofluorimetric Assay

Evaluation of NF-kB Nuclear Translocation in Natural Killer Cells by Imaging Multispectral Flow Cytometry as a Marker of Anticancer Immune Activation

A Colony Formation-Based Assay to Evaluate Natural Killer Cell Antitumor Responses In Vitro

NKT Hybridoma Cell Stimulation Assays to Evaluate Glycolipid Specificity and Processing

Metabolic Profiling of Tumor and Immune Cells Integrating Seahorse and Flow Cytometry

Kinetic Assessment of HMGB1 Exodus by Automated Live Cell Imaging

The Immunogenic Tumor Specific Neoantigen Database (ITSNdb): A Tool for Comprehensive Performance Evaluation of Neoantigen Immunogenicity Predictors

A Robust Quantitative Multiplex PCR for T-Cell Receptor Repertoire Sequencing from Formalin-Fixed Paraffin-Embedded Samples

Immune Single-Cell Annotation by Molecular Signature-Based Deconvolution with MIXTURE

Chromatin Preparation from Frozen Tissues for Chromatin Immunoprecipitation (ChIP) Assays

Exploring the Cancer Immune Epigenome by ATAC-Seq

Epigenomic Profiling of Histone Post-Translational Modifications in Frozen Cancer Tissue Using CUT&RUN

A Luminescence-Based Method for In Vitro Screening of Immunomodulatory Checkpoints and Therapeutics

Generation of Human Monocyte-Derived Immunogenic Dendritic Cells via mTORC1 and PPARy Inhibition

Engineered CAR-NK Cells for Cancer Immunotherapy: Lentiviral-Based CAR Transduction of NK-92 Cells

Optimal Structural Designs of Trispecific Antibodies to Enhance Therapeutic Efficacy in Solid Tumors and Hematological Malignancies

Microbiota Transplantation in Tumor Immunology Studies

Notes:

Online resource; title from PDF title page (SpringerLink, viewed May 28, 2025)

Includes index

Other Format:

Print version :

ISBN:

9781071645581

1071645587

OCLC:

1521243294

Publisher Number:

CIPO000228363

Access Restriction:

Restricted for use by site license